The Effect of Rectangular Electric Pulse Number in Electrochemotherapy by Low Voltage and High Frequency on Breast Tumors in Balb/c Mice

• Published in: Cell journal (Yakhteh) 2010-10, Vol.12 (3), p.381-384
• Main Authors: Shankayi, Zeinab, Firoozabadi, Seyed Mohammad, Hassan, Zahir Mohammad
• Format: Article
• Language: English
• Subjects: Electrochemotherapy; High Frequency; Low Electric Field
• ISSN: 2228-5806; 2228-5814

Objective: The treatment efficiency of electrochemotherapy (ECT) or the use of a chemotherapyagent with a high electric field and low frequency has been reported. Unfortunatelythis protocol induces an unpleasant sensation to the patient. Therefore, the aim of thisstudy was to investigate the efficiency of combined low electric field and high repetitionfrequency for the treatment of an animal tumor model, invasive ductal carcinoma.Materials and Methods: Female Balb/c mice were transplanted with invasive ductal carcinoma.ECT with bleomycin and two different electric pulse simulation protocols wereused. In the first protocol, eight high pulses at an amplitude of 1000 V/cm with 100 μsduration and repetition frequencies of both 5 kHz and 1 Hz were delivered. In the secondprotocol, low pulse amplitude of 100 V/cm with 5 kHz frequency and different numbers ofpulses 500 (1 pulse with 50 milliseconds duration), 2000 (4 pulses with 50 millisecondsduration), 4000 (8 pulses with 50 milliseconds duration) and 5000 (10 pulses with 50 millisecondduration) at 100 μs were applied.Results: ECT with a higher repetition frequency of electric pulses and low voltage inhibitstumor growth and has a comparable effect to the 5 kHz and 1 Hz pulse repetitions athigh voltage. Based on the results, the best antitumor effect was obtained at 4000 pulsesor higher, with high frequency and low voltage (p