Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection

Enterovirus 71 (EV71) is a viral pathogen that belongs to the Picornaviridae family, EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death. In this study, several monoclonal antibodies (MAbs) were produced by immunizing mice with the i...

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Bibliographic Details
Published in:Virology journal 2010-05, Vol.7 (1), p.106-106, Article 106
Main Authors: Chang, Guo-hui, Luo, Yan-jun, Wu, Xiao-yan, Si, Bing-yin, Lin, Lei, Zhu, Qing-yu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - therapeutic use
Antibodies, Viral - immunology
Antibodies, Viral - therapeutic use
Cell Line
Diagnosis
Drug therapy
Enterovirus
Enterovirus A, Human - chemistry
Enterovirus A, Human - genetics
Enterovirus A, Human - immunology
Enterovirus diseases
Enterovirus Infections - immunology
Enterovirus Infections - prevention & control
Enterovirus Infections - therapy
Enterovirus Infections - virology
Enzyme-linked immunosorbent assay
Female
Genetic aspects
Humans
Immunization, Passive
Methods
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Monoclonal antibodies
Picornaviridae
Properties
Sequence Alignment
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - immunology
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
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Summary:Enterovirus 71 (EV71) is a viral pathogen that belongs to the Picornaviridae family, EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death. In this study, several monoclonal antibodies (MAbs) were produced by immunizing mice with the inactived EV71 Henan (Hn2) virus strain. The isolated MAbs were characterised by in vitro neutralizing analysis and peptide ELISA. ELISA assay showed that the neutralizing monoclonal antibody 4E8 specifically reacted with synthetic peptides which contain amino acid 240-250 and 250-260 of EV71 VP1. The in vivo protection assay showed that 4E8 can protect two-day-old BALB/c mice against the lethal challenge of EV71 virus. The MAb 4E8 could be a promising candidate to be humanized and used for treatment of EV71 infection.
ISSN:1743-422X
1743-422X
DOI:10.1186/1743-422X-7-106