Combined Dietary Supplementation of Tenebrio molitor Larvae and Chitosan in Growing Pigs: A Pilot Study

• Published in: Veterinary sciences 2024-02, Vol.11 (2), p.73
• Main Authors: Zacharis, Christos, Bonos, Eleftherios, Voidarou, Chrysoula Chrysa, Magklaras, Georgios, Fotou, Konstantina, Giannenas, Ilias, Giavasis, Ioannis, Mitsagga, Chrysanthi, Athanassiou, Christos, Antonopoulou, Efthimia, Grigoriadou, Katerina, Tzora, Athina, Skoufos, Ioannis
• Format: Article
• Language: English
• Subjects: Amino acids; Animal nutrition; Animal welfare; Animals; Antibiotics; Cell number; chitin; Chitosan; Color; Crustaceans; Diet; Erythrocytes; Fatty acid composition; Fatty acids; Fecal microflora; Feed additives; Feeds; Food; health; Hogs; insect meal; insects; Intestinal microflora; Larvae; Livestock; Lymphocytes; Meals; Meat; Microbiota; Nutrition research; Phenolic compounds; Prebiotics; Proteins; Soybeans; swine; Tenebrio molitor; Weaning
• ISSN: 2306-7381; 2306-7381
• DOI: 10.3390/vetsci11020073

Nowadays, the global animal industry faces considerable challenges in securing sufficient feed resources. Responding to consumer demands for reduced use of antibiotics in animal nutrition, better animal welfare status, and reduced impact on the environment, there is an increased urgency to develop innovative functional feeds with a reduced environmental footprint and the ability to improve meat quality and safety. In an effort to explore innovative feed ingredients for growing pig diets, the combined dietary supplementation of larvae and chitosan was investigated. An experimental trial was performed with 48 weaned pigs (34 days of life; mixed sex) that were randomly assigned to four treatment groups (with six males and six females each): Group A (control), Group B (supplemented with larvae at 10%), Group C (supplemented with chitosan at 0.05%), and Group D (supplemented with both ingredients at 10% and 0.05%, respectively). On the 42nd day of the experimental trial, samples of blood, feces, and carcass parts were taken for analysis. The results indicated that the insect larvae meal significantly improved ( < 0.05) overall performance, increased ( < 0.05) blood red blood cell content, increased meat phenolic content ( < 0.05), improved meat oxidative stability ( < 0.05), and affected meat fatty acid profile ( < 0.05). On the other hand, chitosan had no significant effect on overall performance ( > 0.05), but it significantly increased blood lymphocyte content ( < 0.05), affected the fecal microbiota ( < 0.05), improved meat oxidative stability ( < 0.05), increased meat phenolic content ( < 0.05), and affected meat fatty acid composition ( < 0.05) and ( < 0.05) meat color. Finally, the combined use of both and chitosan significantly affected some important zootechnical parameters ( < 0.05), fecal microbial populations ( < 0.05), meat color ( < 0.05), and fatty acid profile ( < 0.05). Further investigation into the potential interaction between insect larvae meals and chitosan in pig diets is advised.