Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation

Plant cryopreservation technologies are used within gene banks for the long‐term preservation of vegetatively propagated collections. Surface‐sterilized plant tissues grown in the field, greenhouse/screenhouse, growth chamber, or in vitro are the source of shoot tips subjected to vitrification‐based...

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Bibliographic Details
Published in:Applications in plant sciences 2022-09, Vol.10 (5), p.e11489-n/a
Main Authors: Volk, Gayle M., Bonnart, Remi, Oliveira, Annie Carolina Araújo, Henk, Adam D.
Format: Article
Language:English
Subjects:
antibiotic
Bacteria
citrus
Cryopreservation
endophyte
Endophytes
Flowers & plants
Gene banks
in vitro
Microbial contamination
Plant growth
Plant introductions
Plant sciences
Review
Sodium
Tissue culture
Vitrification
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Summary:Plant cryopreservation technologies are used within gene banks for the long‐term preservation of vegetatively propagated collections. Surface‐sterilized plant tissues grown in the field, greenhouse/screenhouse, growth chamber, or in vitro are the source of shoot tips subjected to vitrification‐based cryopreservation methods. Here, we describe the methods used to minimize microbial contamination during the tissue culture initiation process. We also discuss the occurrence and possible elimination of endophytes after extended in vitro culture and during recovery after liquid nitrogen exposure. We describe two case studies in which bacterial endophytes were observed in Citrus gene bank accessions during recovery after cryopreservation. These were identified using the MinION Oxford Nanopore system and Kirby–Bauer disc diffusion assays to examine the bacterial responses to antibiotic exposure. The methods used in this case study could be applied to identify endophytes to better target antimicrobial treatments of plant tissue collections.
ISSN:2168-0450
2168-0450
DOI:10.1002/aps3.11489