New molecular evidence for fragmentation between two distant populations of the threatened stingless bee Melipona subnitida Ducke (Hymenoptera, Apidae, Meliponini)

For a snapshot assessment of the genetic diversity present within Melipona subnitida , an endemic stingless bee distributed in the semi-arid region of northeastern Brazil, populations separated by over 1,000 km distance were analyzed by ISSR genotyping. This is a prerequisite for the establishment o...

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Bibliographic Details
Published in:Journal of Hymenoptera research 2014-06, Vol.38 (38), p.1-9
Main Authors: Silva, Geice R, Souza, Bruno A, Pereira, Fábia M, Lopes, Maria T. R, Valente, Sérgio E. S, Diniz, Fábio M
Format: Article
Language:English
Subjects:
Bees
Biodiversity
Genetic aspects
Genetic diversity
Genotyping
Melipona subnitida
Primers
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Summary:For a snapshot assessment of the genetic diversity present within Melipona subnitida , an endemic stingless bee distributed in the semi-arid region of northeastern Brazil, populations separated by over 1,000 km distance were analyzed by ISSR genotyping. This is a prerequisite for the establishment of efficient management and conservation practices. From 21 ISSR primers tested, only nine revealed consistent and polymorphic bands (loci). PCR reactions resulted in 165 loci, of which 92 were polymorphic (57.5%). Both Φ[sub.ST] (ARLEQUIN) and [THETA][sup.B] (HICKORY) presented high values of similar magnitude (0.34, p
ISSN:1070-9428
1314-2607
DOI:10.3897/JHR.38.7302