Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae

The polyadenosine tail (poly[A]-tail) is a universal modification of eukaryotic messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). In budding yeast, Pap1-synthesized mRNA poly(A) tails enhance export and translation, whereas Trf4/5-mediated polyadenylation of ncRNAs facilitates degradation by the...

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Bibliographic Details
Published in:Nature communications 2021-08, Vol.12 (1), p.4951-4951, Article 4951
Main Authors: Tudek, Agnieszka, Krawczyk, Paweł S., Mroczek, Seweryn, Tomecki, Rafał, Turtola, Matti, Matylla-Kulińska, Katarzyna, Jensen, Torben Heick, Dziembowski, Andrzej
Format: Article
Language:English
Subjects:
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Adenosine
Deprivation
Exonuclease
Gene expression
Gene sequencing
Growth conditions
Humanities and Social Sciences
Metabolism
mRNA
multidisciplinary
Non-coding RNA
Poly(A)
Polyadenylation
Polynucleotide adenylyltransferase
Science
Science (multidisciplinary)
Substrates
Translation
Yeast
Yeasts
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Summary:The polyadenosine tail (poly[A]-tail) is a universal modification of eukaryotic messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). In budding yeast, Pap1-synthesized mRNA poly(A) tails enhance export and translation, whereas Trf4/5-mediated polyadenylation of ncRNAs facilitates degradation by the exosome. Using direct RNA sequencing, we decipher the extent of poly(A) tail dynamics in yeast defective in all relevant exonucleases, deadenylases, and poly(A) polymerases. Predominantly ncRNA poly(A) tails are 20-60 adenosines long. Poly(A) tails of newly transcribed mRNAs are 50 adenosine long on average, with an upper limit of 200. Exonucleolysis by Trf5-assisted nuclear exosome and cytoplasmic deadenylases trim the tails to 40 adenosines on average. Surprisingly, PAN2/3 and CCR4-NOT deadenylase complexes have a large pool of non-overlapping substrates mainly defined by expression level. Finally, we demonstrate that mRNA poly(A) tail length strongly responds to growth conditions, such as heat and nutrient deprivation. RNA polyadenosine tails are important for the export, translation and stability of mRNAs and play a role in non-coding RNA biogenesis. Here the authors measure yeast poly(A) tail lengths by direct RNA sequencing, revealing its dynamics in yeast exonuclease, deadenylase and poly(A) polymerase mutants.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-25251-w