Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological...

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Bibliographic Details
Published in:Marine drugs 2018-08, Vol.16 (8), p.287
Main Authors: Tihan, Gratiela Teodora, Zgarian, Roxana Gabriela, Berteanu, Elena, Ionita, Daniela, Totea, Georgeta, Iordachel, Catalin, Tatia, Rodica, Prodana, Mariana, Demetrescu, Ioana
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Analytical methods
Bacteria
Biocompatibility
Biomedical materials
Biotechnology
cell viability
Chitosan
Composition
E coli
Electron microscopy
Enzymatic activity
Enzyme activity
Fabrication
Fourier transforms
Haemolysis
hemolytic index
Immobilization
Inductively coupled plasma mass spectrometry
Infrared spectroscopy
Ions
Magnesium
Mass spectrometry
Mass spectroscopy
Membranes
metal ion
Metal ions
Metals
Organic chemistry
Phosphatase
Physical characteristics
Scanning electron microscopy
Supports
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Summary:In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+. The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.
ISSN:1660-3397
1660-3397
DOI:10.3390/md16080287