Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand

• Published in: Microorganisms (Basel) 2023-11, Vol.11 (12), p.2855
• Main Authors: Dokuta, Sirikwan, Yadoung, Sumed, Jeeno, Peerapong, Hongjaisee, Sayamon, Khamnoi, Phadungkiat, Manochomphu, Sirinya, Danmek, Khanchai, Maitip, Jakkrawut, Chuttong, Bajaree, Hongsibsong, Surat
• Format: Article
• Language: English
• Subjects: Acinetobacter; Agriculture; Antibiotic resistance; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Bacteria; beehive; Beehives; Beekeeping; Bees; Biochemical tests; Cefotaxime; Cefoxitin; Ceftazidime; Ciprofloxacin; Corn; Drug resistance; Drug resistance in microorganisms; Environmental aspects; Epidemics; Fungi; Gram-negative bacteria; Gram-positive bacteria; Health aspects; Honey; Identification; Identification and classification; Imipenem; Levofloxacin; Medical laboratories; Meropenem; Microorganisms; Organisms; Palms; Pathogenic microorganisms; Plantations; plantations in Thailand; Pollinators; Public health; Risk factors; Soapberries; Transport media; Vancomycin; Yeast; Yeasts
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11122855

This study aims to determine the prevalence of microorganisms and antibiotic-resistant microorganisms in beehives located on different plantations in Thailand. Seventeen swabs immersed in transport media were utilized for samples from different zones within beehives. Traditional microbial culture-based methods, biochemical tests, MALDI-TOF MS (VITEK MS, bioMerieux, Marcy-l'Étoile, France), and antibiotic drug susceptibility (disk-diffusion) tests were used to detect microorganism and antimicrobial resistance bacteria. The results from 16 beehive swabs found Gram-positive bacteria at 59.5%, Gram-negative bacteria at 35.1%, and fungi (yeast) at 5.4%. These organisms are classified as 11, 11, and 2 types of Gram-positive bacteria, Gram-negative bacteria, and fungi (yeast), respectively. Furthermore, no organism showed resistance to vancomycin or cefoxitin for antibiotic drug susceptibility testing. In contrast, all spp. were susceptible to ciprofloxacin, levofloxacin, ceftazidime, cefotaxime, imipenem, and meropenem, except for , which was resistant to ceftazidime and cefotaxime. For other organisms, due to the limitations of tests to identify some environmental microbial species, the antimicrobial susceptibility test results cannot be interpreted as resistant or susceptible to the drug for these organisms. The study's findings will support prevention, healthcare services, and public health systems.