Comparison of USA300 with non-USA300 methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit

•Our study compared USA300 with non-USA300 MRSA in a NICU.•MRSA detected from NICU were analyzed by molecular methods.•Significant difference was not observed for a proportion of clinical diseases.•Significant difference was not observed for mortality rates. Reports of USA300 methicillin-resistant S...

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Published in:International journal of infectious diseases 2019-02, Vol.79, p.134-138
Main Authors: Murai, Takemi, Okazaki, Kaoru, Kinoshita, Kazue, Uehara, Yuki, Zuo, Hui, Lu, Yujie, Ono, Yuki, Sasaki, Takashi, Hiramatsu, Keiichi, Horikoshi, Yuho
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Language:English
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Arginine catabolic mobile element
Methicillin-resistant Staphylococcus aureus
Neonatal intensive care unit
Panton-Valentine leukocidin
USA300
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container_title International journal of infectious diseases
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creator Murai, Takemi
Okazaki, Kaoru
Kinoshita, Kazue
Uehara, Yuki
Zuo, Hui
Lu, Yujie
Ono, Yuki
Sasaki, Takashi
Hiramatsu, Keiichi
Horikoshi, Yuho
description •Our study compared USA300 with non-USA300 MRSA in a NICU.•MRSA detected from NICU were analyzed by molecular methods.•Significant difference was not observed for a proportion of clinical diseases.•Significant difference was not observed for mortality rates. Reports of USA300 methicillin-resistant Staphylococcus aureus (MRSA) strain were still scarce in neonatal intensive care units (NICUs) and the relationship of USA300 MRSA to clinical infections is still controversial. The primary outcome was the incidence of MRSA infections caused by the USA300 and non-USA300 strains at a NICU in Japan. This retrospective cohort study was conducted between November 2011 and October 2016 at Tokyo Metropolitan Children’s Medical Center in Japan. All MRSA isolated after 48h of hospitalization were included for analysis by pulsed-field gel electrophoresis (PFGE) using the standard USA300 strain. Genes were tested for Panton-Valentine leukocidin (PVL) and arginine catabolic mobile element (ACME). A whole genome sequence was performed for representative isolates of USA300. In total, 109 MRSA isolates were included for analysis. PFGE classified 34 and 75 isolates of USA300 and non-USA300 MRSA, respectively. Both PVL and ACME genes were detected in USA300 and non-USA300 strains at rate of 100% (34/34) and 5.3% (4/75), respectively (P
doi_str_mv 10.1016/j.ijid.2018.11.020
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Reports of USA300 methicillin-resistant Staphylococcus aureus (MRSA) strain were still scarce in neonatal intensive care units (NICUs) and the relationship of USA300 MRSA to clinical infections is still controversial. The primary outcome was the incidence of MRSA infections caused by the USA300 and non-USA300 strains at a NICU in Japan. This retrospective cohort study was conducted between November 2011 and October 2016 at Tokyo Metropolitan Children’s Medical Center in Japan. All MRSA isolated after 48h of hospitalization were included for analysis by pulsed-field gel electrophoresis (PFGE) using the standard USA300 strain. Genes were tested for Panton-Valentine leukocidin (PVL) and arginine catabolic mobile element (ACME). A whole genome sequence was performed for representative isolates of USA300. In total, 109 MRSA isolates were included for analysis. PFGE classified 34 and 75 isolates of USA300 and non-USA300 MRSA, respectively. Both PVL and ACME genes were detected in USA300 and non-USA300 strains at rate of 100% (34/34) and 5.3% (4/75), respectively (P&lt;0.05). There was no statistically significant difference in the proportion of clinical diseases between USA- 300 and non-USA 300 strains. Infants with USA300 MRSA infection did not differ significantly from those with non-USA300 MRSA infection.</description><identifier>ISSN: 1201-9712</identifier><identifier>EISSN: 1878-3511</identifier><identifier>DOI: 10.1016/j.ijid.2018.11.020</identifier><identifier>PMID: 30503654</identifier><language>eng</language><publisher>Canada: Elsevier Ltd</publisher><subject>Arginine catabolic mobile element ; Methicillin-resistant Staphylococcus aureus ; Neonatal intensive care unit ; Panton-Valentine leukocidin ; USA300</subject><ispartof>International journal of infectious diseases, 2019-02, Vol.79, p.134-138</ispartof><rights>2018 The Author(s)</rights><rights>Copyright © 2018 The Author(s). Published by Elsevier Ltd.. 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subjects Arginine catabolic mobile element
Methicillin-resistant Staphylococcus aureus
Neonatal intensive care unit
Panton-Valentine leukocidin
USA300
title Comparison of USA300 with non-USA300 methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit
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