Comparative Metabolomics Analysis of Stigmas and Petals in Chinese Saffron ( Crocus sativus ) by Widely Targeted Metabolomics

• Published in: Plants (Basel) 2022-09, Vol.11 (18), p.2427
• Main Authors: Zhou, Lin, Cai, Youming, Yang, Liuyan, Zou, Zhongwei, Zhu, Jiao, Zhang, Yongchun
• Format: Article
• Language: English
• Subjects: Alkaloids; Amino acids; Annotations; Biosynthesis; Carbohydrates; Chemical properties; Chromatography; Coumarins; Crocus sativus; Daidzein; Discriminant analysis; Esters; Flavonoids; Flowers; Food; Glucosides; Health drinks; Hydrocarbons; Isoflavones; Lipids; Mass spectrometry; Medicinal plants; Metabolism; Metabolites; Metabolomics; metabolomics analysis; Metabonomic analysis; Petals; Phenols; Phenylpropanoids; Physiological aspects; Plant metabolites; Principal components analysis; Product development; Proline; Quercetin; Research methodology; Rutin; Saffron; Saffron crocus; Scientific imaging; Steroid hormones; Steroids; Stigma; stigmas; Stigmas (botany); Vitamins
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11182427

The dried stigmas of , commonly known as saffron, are consumed largely worldwide because it is highly valuable in foods and has biological activities beneficial for health. Saffron has important economic and medicinal value, and thus, its planting area and global production are increasing. Petals, which are a by-product of the stigmas, have not been fully utilized at present. We compared the metabolites between the stigmas and petals of using a non-targeted metabolomics method. In total, over 800 metabolites were detected and categorized into 35 classes, including alkaloids, flavonoids, amino acids and derivatives, phenols and phenol esters, phenylpropanoids, fatty acyls, steroids and steroid derivatives, vitamins, and other metabolites. The metabolite composition in the petals and stigmas was basically similar. The results of the study showed that the petals contained flavonoids, alkaloids, coumarins, and other medicinal components, as well as amino acids, carbohydrates, vitamins, and other nutritional components. A principal components analysis (PCA) and an orthogonal partial least-squares discriminant analysis (OPLS-DA) were performed to screen the different metabolic components. A total of 339 differential metabolites were identified, with 55 metabolites up-regulated and 284 down-regulated. The up-regulated metabolites, including rutin, delphinidin-3- -glucoside, isoquercitrin, syringaresinol-di- -glucoside, dihydrorobinetin, quercetin, and gallocatechin, were detected in the petals. The down-regulated metabolites were mainly glucofrangulin B, acetovanillone, daidzein, guaiazulene, hypaphorine, indolin-2-one, and pseudouridine. KEGG annotation and enrichment analyses of the differential metabolites revealed that flavonoid biosynthesis, amino acids biosynthesis, and arginine and proline metabolism were the main differentially regulated pathways. In conclusion, the petals of are valuable for medicine and foods and have potential utility in multiple areas such as the natural spice, cosmetic, health drink, and natural health product industries.