Early patterning and specification of cardiac progenitors in gastrulating mesoderm

Mammalian heart development requires precise allocation of cardiac progenitors. The existence of a multipotent progenitor for all anatomic and cellular components of the heart has been predicted but its identity and contribution to the two cardiac progenitor 'fields' has remained undefined...

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Bibliographic Details
Published in:eLife 2014-10, Vol.3
Main Authors: Devine, W Patrick, Wythe, Joshua D, George, Matthew, Koshiba-Takeuchi, Kazuko, Bruneau, Benoit G
Format: Article
Language:English
Subjects:
Animals
Basic Helix-Loop-Helix Transcription Factors - metabolism
Body Patterning
Cell fate
Chromosomal Proteins, Non-Histone - metabolism
Clone Cells
Cloning
Confidence intervals
Developmental Biology and Stem Cells
embryo
Embryo, Mammalian - metabolism
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Enhancer Elements, Genetic - genetics
Fate maps
Gastrulation
Gene mapping
Heart
Heart - embryology
Heart diseases
Heart Ventricles - embryology
Heart Ventricles - metabolism
Integrases - metabolism
Labeling
lineage
Mesoderm
Mesoderm - cytology
Mesoderm - embryology
Mice
Models, Biological
Morphogenesis
Muscle Proteins - metabolism
Myocardium - cytology
Organ Specificity - genetics
Population
Stem cells
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Summary:Mammalian heart development requires precise allocation of cardiac progenitors. The existence of a multipotent progenitor for all anatomic and cellular components of the heart has been predicted but its identity and contribution to the two cardiac progenitor 'fields' has remained undefined. Here we show, using clonal genetic fate mapping, that Mesp1+ cells in gastrulating mesoderm are rapidly specified into committed cardiac precursors fated for distinct anatomic regions of the heart. We identify Smarcd3 as a marker of early specified cardiac precursors and identify within these precursors a compartment boundary at the future junction of the left and right ventricles that arises prior to morphogenesis. Our studies define the timing and hierarchy of cardiac progenitor specification and demonstrate that the cellular and anatomical fate of mesoderm-derived cardiac cells is specified very early. These findings will be important to understand the basis of congenital heart defects and to derive cardiac regeneration strategies.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.03848