Effect of nano-particles of zinc oxide and selenium on antioxidant status, aminotransferase enzymes activities and genes expression of sod-1 and vg in honey bee during the hot season

• Published in: Journal of Trace Elements and Minerals (Online) 2022-12, Vol.2, p.100034, Article 100034
• Main Authors: Amini-Esfidvajani, Mohammad-Bagher, Sadeghi, Ali Asghar, Shawrang, Parvin, Chamani, Mohammad, Aminafshar, Mehdi
• Format: Article
• Language: English
• Subjects: Body weight; Brood; Heat stress; Malondialdehyde; Vitellogenin
• ISSN: 2773-0506; 2773-0506
• DOI: 10.1016/j.jtemin.2022.100034

The nano-particle of zinc oxide (nZn) and selenium (nSe) has recently attracted in animal feeding because of their high absorbability, catalytic efficiency, and low toxicity than organic and non-organic forms. The present study was done to evaluate the effect of nZn and nSe supplementation in the honey bee's diet under heat stress on antioxidant status, aminotransferase activity, and the relative gene expression of vg and sod-1. Fifty honey bee colonies were randomly divided into five treatments and 10 replicates. Treatments were sugar syrup without or with nanoparticle supplementation, as: i) the control group (no additives); ii) 100 µgL−1 nano-zinc oxide (nZn-1); iii) 200 µg L − 1 of nano-zinc oxide (nZn-2); iv) 100 µg L − 1 nano-selenium (nSe-1); v) 200 µg L − 1 nano-selenium (nSe-2). On days 30 and 60 of experiment, the bee's samples were taken and antioxidant status, aminotransferase activities and relative genes expression of vg and sod-1 were measured. The highest (P