Simultaneous determination of meloxicam and bupivacaine via a novel modified dual wavelength method and an advanced chemometric approach

This study presents two spectrophotometric methods; a novel dual wavelength—derivative spectrophotometry and multivariate curve resolution-alternating least squares (MCR-ALS) for the simultaneous determination of a fixed dose combination of bupivacaine (BUP) and meloxicam (MEL) in a ratio of 30:1. T...

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Bibliographic Details
Published in:Scientific reports 2024-01, Vol.14 (1), p.1893-9, Article 1893
Main Authors: El-Malla, Samah F., Hamza, Aliaa A., Elagamy, Samar H.
Format: Article
Language:English
Subjects:
639/638/11
639/638/224
Bupivacaine
Calibration
Chemometrics
Chromatography, High Pressure Liquid
Drug dosages
Humanities and Social Sciences
Liquid chromatography
Meloxicam
Methods
multidisciplinary
Pharmaceuticals
Science
Science (multidisciplinary)
Sodium
Software
Solvents
Spectrophotometry
Ultraviolet radiation
Wavelength
Wavelengths
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Summary:This study presents two spectrophotometric methods; a novel dual wavelength—derivative spectrophotometry and multivariate curve resolution-alternating least squares (MCR-ALS) for the simultaneous determination of a fixed dose combination of bupivacaine (BUP) and meloxicam (MEL) in a ratio of 30:1. The extended UV spectrum of MEL enables its direct determination at λ max 360 nm with no interference from BUP. The determination of BUP was unfeasible directly because the UV spectra of both drugs are moderately overlapped over the wavelength range of 250–450 nm, thus new chemometric based spectrophotometric methods should be developed for its determination. Dual wavelength-derivative method was employed based on using first derivative spectra. The selected dual wavelengths for determination BUP were 274.6 nm and 374.6 nm where the dA/dλ amplitudes differences for MET are equal to zero. MCR-ALS is advanced chemometric tool that enables analysis of multicomponent samples in complex matrices with high resolution based on the decomposition of signal/spectral data into the pure spectra and corresponding concentration profile. The figures of merits for MCR model show that there is a good agreement between the actual and predicted concentrations for MEL and BUP. The methods were validated and statistically compared with a reported HPLC method.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-51885-z