Detection of the antibiotic resistance genes content of intestinal Bacteroides, Parabacteroides and Phocaeicola isolates from healthy and carbapenem-treated patients from European countries

• Published in: BMC microbiology 2024-06, Vol.24 (1), p.202-9
• Main Authors: Sóki, József, Wybo, Ingrid, Baaity, Zain, Stefán, Glória, Jeverica, Samo, Ulger, Nurver, Stingu, Catalina-Suzana, Mahmood, Bakhtiyar, Burián, Katalin, Nagy, Elisabeth
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotic resistance genes; Antibiotics; Antimicrobial resistance; Bacterial infections; Bacterial Proteins - genetics; Bacteroides; Bacteroides - drug effects; Bacteroides - genetics; Bacteroides - isolation & purification; Bacteroides Infections - microbiology; Beta lactamases; Carbapenems; Carbapenems - pharmacology; Care and treatment; Diagnosis; Drug resistance; Drug resistance in microorganisms; Drug Resistance, Bacterial - genetics; Europe; Gastrointestinal Microbiome - drug effects; Gastrointestinal Microbiome - genetics; Genes; Genes, Bacterial - genetics; Gut lumen; Health aspects; Humans; Imipenem; Infection; Infections; Intestinal microflora; Intestine; Intestines - microbiology; Medical colleges; Microbial Sensitivity Tests; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Parabacteroides; Pathogens; Phocaeicola; Surveys; Tetracycline; Tetracyclines; β Lactamase
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-024-03354-w

Bacteroides fragilis group (BFG) species are the most significant anaerobic pathogens and are also the most antibiotic-resistant anaerobic species. Therefore, surveying their antimicrobial resistance levels and investigating their antibiotic resistance mechanisms is recommended. Since their infections are endogenous and they are important constituents of the intestinal microbiota, the properties of the intestinal strains are also important to follow. The aim of this study was to investigate the main antibiotic gene content of microbiota isolates from healthy people and compare them with the gene carriage of strains isolated from infections. We detected 13, mainly antibiotic resistance determinants of 184 intestinal BFG strains that were isolated in 5 European countries (Belgium, Germany, Hungary, Slovenia and Turkey) and compared these with values obtained earlier for European clinical strains. Differences were found between the values of this study and an earlier one for antibiotic resistance genes that are considered to be mobile, with higher degrees for cfxA, erm(F) and tet(Q) and with lower degrees for msrSA, erm(B) and erm(G). In addition, a different gene prevalence was found depending on the taxonomical groups, e.g., B. fragilis and NBFB. Some strains with both the cepA and cfiA β-lactamase genes were also detected, which is thought to be exceptional since until now, the B. fragilis genetic divisions were defined by the mutual exclusion of these two genes. Our study detected the prevalences of a series of antibiotic resistance genes in intestinal Bacteroides strains which is a novelty. In addition, based on the current and some previous data we hypothesized that prevalence of some antibiotic resistance genes detected in the clinical and intestinal BFG strains were different, which could be accounted with the differential composition of the Bacteroides microbiota and/or the MGE mobilities at the luminal vs. mucosal sites of the intestine.