Plasma Amino Acid Response to Whey Protein Ingestion Following 28 Days of Probiotic ( Bacillus subtilis DE111) Supplementation in Active Men and Women

We sought to determine if 28 days of probiotic supplementation influenced the plasma amino acid (AA) response to acute whey protein feeding. Twenty-two recreationally active men ( = 11; 24.3 ± 3.2 yrs; 89.3 ± 7.2 kg) and women ( = 11; 23.0 ± 2.8 yrs; 70.2 ± 15.2 kg) participated in this double-blind...

Full description

Saved in:
Bibliographic Details
Published in:Journal of functional morphology and kinesiology 2020-12, Vol.6 (1), p.1-0
Main Authors: Townsend, Jeremy R, Vantrease, William C, Jones, Megan D, Sapp, Philip A, Johnson, Kent D, Beuning, Cheryle N, Haase, Allison A, Boot, Claudia M
Format: Article
Language:English
Subjects:
absorption
Amino acids
BCAA
Body composition
Body fat
Exercise
Food
gut
Laboratories
leucine
Mens health
microbiome
Plasma
Probiotics
protein synthesis
Proteins
Tumor necrosis factor-TNF
Women
Womens health
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We sought to determine if 28 days of probiotic supplementation influenced the plasma amino acid (AA) response to acute whey protein feeding. Twenty-two recreationally active men ( = 11; 24.3 ± 3.2 yrs; 89.3 ± 7.2 kg) and women ( = 11; 23.0 ± 2.8 yrs; 70.2 ± 15.2 kg) participated in this double-blind, placebo-controlled, randomized study. Before (PRE) and after 28 days of supplementation (POST), participants reported to the lab following a 10-hr fast and provided a resting blood draw (0 min), then subsequently consumed 25 g of whey protein. Blood samples were collected at 15-min intervals for 2 h post-consumption (15-120 min) and later analyzed for plasma leucine, branched-chain AA (BCAA), essential AA (EAA), and total AA (TAA). Participants received a probiotic (PROB) consisting of 1 x10-9 colony forming units (CFU) DE111 ( = 11) or a maltodextrin placebo (PL) ( = 11) for 28 days. Plasma AA response and area under the curve (AUC) values were analyzed via repeated measures analysis of variance. Our analysis indicated no significant ( < 0.05) differential responses for plasma leucine, BCAA, EAA, or TAA between PROB and PL from PRE to POST. AUC analysis revealed no group × time interaction for plasma leucine ( = 0.524), BCAA ( = 0.345), EAA ( = 0.512), and TAA ( = 0.712). These data indicate that 28 days of DE111 does not affect plasma AA appearance following acute whey protein ingestion.
ISSN:2411-5142
2411-5142
DOI:10.3390/jfmk6010001