Rapid detection of high-level oncogene amplifications in ultrasonic surgical aspirations of brain tumors

Genomic tumor information, such as identification of amplified oncogenes, can be used to plan treatment. The two sources of a brain tumor that are commonly available include formalin-fixed, paraffin-embedded (FFPE) sections from the small diagnostic biopsy and the ultrasonic surgical aspiration that...

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Bibliographic Details
Published in:Diagnostic pathology 2012-06, Vol.7 (1), p.66-66, Article 66
Main Authors: Truong, Long N, Patil, Shashikant, Martin, Sherry S, LeBlanc, Jay F, Nanda, Anil, Nordberg, Mary L, Beckner, Marie E
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - genetics
Biopsy, Needle
Brain
Brain cancer
Brain Neoplasms - genetics
Brain Neoplasms - secondary
Brain Neoplasms - surgery
Brain tumors
Cancer
Centrifugation
Comparative analysis
Deoxyribonucleic acid
DNA
Ependymoma - genetics
Ependymoma - pathology
Ependymoma - surgery
Female
Fixatives
Formaldehyde
Gene Amplification
Genes
Genetic aspects
Genetic testing
Glioblastoma - genetics
Glioblastoma - pathology
Glioblastoma - surgery
Health sciences
Hospitals
Humans
In Situ Hybridization, Fluorescence
Lung cancer
Lung Neoplasms - genetics
Lung Neoplasms - secondary
Male
Medicine
Metastasis
Methodology
Middle Aged
Multiplex Polymerase Chain Reaction
Neurosurgery
Oncogenes
Paraffin Embedding
Pathology
Predictive Value of Tests
Proteins
Receptor, Epidermal Growth Factor - genetics
Reproducibility of Results
Review boards
Studies
Surgery
Time Factors
Tissue Fixation
Tumors
Ultrasonic Surgical Procedures
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Summary:Genomic tumor information, such as identification of amplified oncogenes, can be used to plan treatment. The two sources of a brain tumor that are commonly available include formalin-fixed, paraffin-embedded (FFPE) sections from the small diagnostic biopsy and the ultrasonic surgical aspiration that contains the bulk of the tumor. In research centers, frozen tissue of a brain tumor may also be available. This study compared ultrasonic surgical aspiration and FFPE specimens from the same brain tumors for retrieval of DNA and molecular assessment of amplified oncogenes. Surgical aspirations were centrifuged to separate erythrocytes from the tumor cells that predominantly formed large, overlying buffy coats. These were sampled to harvest nuclear pellets for DNA purification. Four glioblastomas, 2 lung carcinoma metastases, and an ependymoma were tested. An inexpensive PCR technique, multiplex ligation-dependent probe amplification (MLPA), quantified 79 oncogenes using 3 kits. Copy number (CN) results were normalized to DNA from non-neoplastic brain (NB) in calculated ratios, [tumor DNA]/[NB DNA]. Bland-Altman and Spearman rank correlative comparisons were determined. Regression analysis identified outliers. Purification of DNA from ultrasonic surgical aspirations was rapid (
ISSN:1746-1596
1746-1596
DOI:10.1186/1746-1596-7-66