Utilization of DNA microarrays for detection and identification of selected Fusarium species from the Czech Republic

Fusarium is a serious phytopathogenic fungal genus with producting of many kinds of highly toxic secondary metabolites – mycotoxins. The consumption of Fusarium contaminated food and feed can cause dangerous mycotoxicoses both in humans and animals, therefore the detection of a wider range of Fusari...

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Bibliographic Details
Published in:Czech Journal of Food Sciences 2011-01, Vol.29 (Special Issue), p.S93-S101
Main Authors: Pavlátová, Lucie, Novotný, David, Hodek, Jan, Chrpová, Jana, Ovesná, Jaroslava
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
DNA chips
DNA microarrays
DNA probes
Elongation
Fluorescence
fluorescent labelling
Food contamination
Fungi
Fusarium
Hybridization
Metabolites
Mycotoxins
oligonucleotide probe
Oligonucleotides
pcr
Polymerase chain reaction
Secondary metabolites
Species
Translation elongation
Wheat
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Summary:Fusarium is a serious phytopathogenic fungal genus with producting of many kinds of highly toxic secondary metabolites – mycotoxins. The consumption of Fusarium contaminated food and feed can cause dangerous mycotoxicoses both in humans and animals, therefore the detection of a wider range of Fusarium species in the samples of crops is very important. The aim of our work was to test the reliability of detection and identification of three Fusarium species in infected wheat grains by DNA microarrays versus classical mycological methods and by specific PCR. The in-house DNA microarrays for the detection and identification of the selected Fusarium species by using oligonucleotides probes were prepared. For hybridisation on DNA microarrays fluorescent labelled PCR products were used of part of the translation elongation factor 1 alpha. The conditions of hybridisation were optimised on fungal template DNA. The method of DNA microarrays was verified on artificially infected samples of wheat and tested on unknown infected wheat samples with simultaneous analysis by classical mycological methods and by specific PCR.
ISSN:1212-1800
1805-9317
DOI:10.17221/307/2011-CJFS