Fluorescence In Situ Hybridization for Diagnosis of Whipple's Disease in Formalin-Fixed Paraffin-Embedded Tissue

Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. The aim of our...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in medicine 2017-06, Vol.4, p.87-87
Main Authors: Braubach, Peter, Lippmann, Torsten, Raoult, Didier, Lagier, Jean-Christophe, Anagnostopoulos, Ioannis, Zender, Steffen, Länger, Florian Peter, Kreipe, Hans-Heinrich, Kühnel, Mark Philipp, Jonigk, Danny
Format: Article
Language:English
Subjects:
fluorescence in situ hybridization
formalin-fixed paraffin-embedded tissue
Human health and pathology
immunohistochemistry
Life Sciences
Medicine
Surgery
Tropheryma whipplei
Whipple’s disease
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. The aim of our study was to improve the pathological diagnosis of WD. Therefore, we analyzed the potential of fluorescence hybridization (FISH) for diagnosing WD, using a -specific probe. 19 formalin-fixed paraffin-embedded (FFPE) duodenal biopsy specimens of 12 patients with treated (6/12) and untreated (6/12) WD were retrospectively examined using PAS diastase staining, immunohistochemistry, and FISH. 20 biopsy specimens with normal intestinal mucosa, , or mycobacterial infection, respectively, served as controls. We successfully detected in tissue biopsies with a sensitivity of 83% in untreated (5/6) and 40% in treated (4/10) cases of WD. In our study, we show that FISH-based diagnosis of individual vital in FFPE specimens is feasible and can be considered as ancillary diagnostic tool for the diagnosis of WD in FFPE material. We show that FISH not only detect active WD but also be helpful as an indicator for the efficiency of antibiotic treatment and for detection of recurrence of disease when the signal of PAS diastase and immunohistochemistry lags behind the recurrence of disease, especially if the clinical course of the patient and antimicrobial treatment is considered.
ISSN:2296-858X
2296-858X
DOI:10.3389/fmed.2017.00087