Miniaturized Rapid Electrochemical Immunosensor Based on Screen Printed Carbon Electrodes for Mycobacterium tuberculosis Detection

In 2019, over 21% of an estimated 10 million new tuberculosis (TB) patients were either not diagnosed at all or diagnosed without being reported to public health authorities. It is therefore critical to develop newer and more rapid and effective point-of-care diagnostic tools to combat the global TB...

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Bibliographic Details
Published in:Biosensors (Basel) 2023-05, Vol.13 (6), p.589
Main Authors: Zouaghi, Noura, Aziz, Shahid, Shah, Imran, Aamouche, Ahmed, Jung, Dong-Won, Lakssir, Brahim, Ressami, El Mostafa
Format: Article
Language:English
Subjects:
Assaying
Biosensing Techniques
Carbon
carbon screen-printed electrode
COVID-19
CV analysis
Deoxyribonucleic acid
DNA
Electrochemistry
Electrodes
Epidemics
Gene amplification
Humans
Immunoassay
Immunosensors
Infectious diseases
LAMP-Electrochemical
Mortality
Mycobacterium tuberculosis
Nucleic Acid Amplification Techniques - methods
Nucleic acids
Nucleotide sequence
Public health
Sensitivity and Specificity
Sensors
Thermal cycling
Tuberculosis
Tuberculosis - diagnosis
Tuberculosis - microbiology
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Summary:In 2019, over 21% of an estimated 10 million new tuberculosis (TB) patients were either not diagnosed at all or diagnosed without being reported to public health authorities. It is therefore critical to develop newer and more rapid and effective point-of-care diagnostic tools to combat the global TB epidemic. PCR-based diagnostic methods such as Xpert MTB/RIF are quicker than conventional techniques, but their applicability is restricted by the need for specialized laboratory equipment and the substantial cost of scaling-up in low- and middle-income countries where the burden of TB is high. Meanwhile, loop-mediated isothermal amplification (LAMP) amplifies nucleic acids under isothermal conditions with a high efficiency, helps in the early detection and identification of infectious diseases, and can be performed without the need for sophisticated thermocycling equipment. In the present study, the LAMP assay was integrated with screen-printed carbon electrodes and a commercial potentiostat for real time cyclic voltammetry analysis (named as the LAMP-Electrochemical (EC) assay). The LAMP-EC assay was found to be highly specific to TB-causing bacteria and capable of detecting even a single copy of the (Mtb) IS DNA sequence. Overall, the LAMP-EC test developed and evaluated in the present study shows promise to become a cost-effective tool for rapid and effective diagnosis of TB.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13060589