A Multi-Site Assessment of Anesthetic Overdose, Hypothermic Shock, and Electrical Stunning as Methods of Euthanasia for Zebrafish ( Danio rerio ) Embryos and Larvae

Euthanasia in zebrafish (Danio rerio) younger than 5 days post fertilization (dpf) is poorly described in the literature, and standardized protocols are lacking, most likely because larvae not capable of independent feeding are often not protected under national legislations. We assessed the euthana...

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Published in:Biology (Basel, Switzerland) Switzerland), 2022-04, Vol.11 (4), p.546
Main Authors: Mocho, Jean-Philippe, Lang, Florian, Valentin, Guillaume, Bedu, Sébastien, McKimm, Robin, Ramos, Juan, Saavedra Torres, Yolanda, Wheatley, Sarah E, Higgins, Joseph, Millington, Mollie E, Lundegaard, Pia Rengtved, Chamorro Valverde, Rubén, Jenčič, Vlasta, von Krogh, Kristine
Format: Article
Language:English
Subjects:
anesthesia
Anesthetics
Animal welfare
Aversion
Consciousness
Danio rerio
Developmental stages
Embryos
Essential oils
Ethanol
euthanasia
Experiments
Fertilization
Immersion
Laboratories
Larvae
Legislation
Lidocaine
Nitrates
Overdose
Phenoxyethanol
Sodium bicarbonate
Solvents
Survival
tricaine
Zebrafish
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Summary:Euthanasia in zebrafish (Danio rerio) younger than 5 days post fertilization (dpf) is poorly described in the literature, and standardized protocols are lacking, most likely because larvae not capable of independent feeding are often not protected under national legislations. We assessed the euthanasia efficacy in laboratories in different countries of a one hour anesthetic overdose immersion with buffered lidocaine hydrochloride (1 g/L, with or without 50 mL/L of ethanol), buffered tricaine (1 g/L), clove oil (0.1%), benzocaine (1 g/L), or 2-phenoxyethanol (3 mL/L), as well as the efficacy of hypothermic shock (one hour immersion) and electrical stunning (for one minute), on zebrafish at
ISSN:2079-7737
2079-7737
DOI:10.3390/biology11040546