Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum

A simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample. Samples were pretreated by a single-step precipitation with acetonitrile....

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2020, Vol.2020 (2020), p.1-10
Main Authors: Liu, Yanpeng, Zhang, Qiao, Hu, Xingjiang, Xu, Nana, Zheng, Yunliang, Zhao, Qingwei
Format: Article
Language:English
Subjects:
Acetonitrile
Analytical chemistry
Antitubercular agents
Chromatography
Clinical medicine
Complications and side effects
Drug resistance
Drugs
Elution
Ionization
Ions
Linearity
Liquid chromatography
Mass spectrometry
Methods
Monitoring
Nitriles
Quadrupoles
Scientific imaging
Solvents
Tuberculosis
Type 2 diabetes
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Summary:A simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample. Samples were pretreated by a single-step precipitation with acetonitrile. Chromatographic separation was achieved on XSelecT HSS T3 column by gradient elution with a total run time of 5.0 min. MS detection was performed by a triple quadrupole tandem mass spectrometer in the multiple reaction monitoring mode with a positive electrospray ionization source. Isotope-labeled internal standard, especially rifampicin-D8, was applied to adjust for the loss during sample treatment. The established LC-MS/MS method showed a wide analytical range (pyrazinamide: 1.02∼60.0 μg/mL, isoniazid: 0.152∼10.0 μg/mL, rifampicin: 0.500∼30.0 μg/mL, and ethambutol: 0.0998∼5.99 μg/mL) and a good linearity (r > 0.99 for the four analytes) with acceptable accuracy and precision (90.15%∼104.62% and 94.00%∼104.02% for intra- and interaccuracy, respectively; RSD%:
ISSN:2090-8865
2090-8873
DOI:10.1155/2020/8838219