Loading…

Microbiome features in bronchoalveolar lavage fluid of patients with idiopathic inflammatory myopathy-related interstitial lung disease

Interstitial lung disease (ILD) is a common complication of idiopathic inflammatory myopathy (IIM), which is one of the connective tissue diseases (CTD). It can lead to poor prognosis and increased mortality. However, the distribution and role of the lower respiratory tract (LRT) microbiome in patie...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in medicine 2024-04, Vol.11, p.1338947-1338947
Main Authors: Zhang, Liyan, Liu, Xueqing, Fan, Bijun, Chen, Jiajun, Chen, Jie, Li, Qiuhong, Wu, Xueling
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c418t-bf345962f889f03cb95631940d41d13e601aa5a6fc5e4f77f3ef8d8871219c8e3
container_end_page 1338947
container_issue
container_start_page 1338947
container_title Frontiers in medicine
container_volume 11
creator Zhang, Liyan
Liu, Xueqing
Fan, Bijun
Chen, Jiajun
Chen, Jie
Li, Qiuhong
Wu, Xueling
description Interstitial lung disease (ILD) is a common complication of idiopathic inflammatory myopathy (IIM), which is one of the connective tissue diseases (CTD). It can lead to poor prognosis and increased mortality. However, the distribution and role of the lower respiratory tract (LRT) microbiome in patients with IIM-ILD remains unclear. This study aimed to investigate the microbial diversity and community differences in bronchoalveolar lavage fluid (BALF) in patients with IIM-ILD. From 28 June 2021 to 26 December 2023, 51 individual BALF samples were enrolled, consisting of 20 patients with IIM-ILD, 16 patients with other CTD-ILD (including 8 patients with SLE and 8 with RA) and 15 patients with CAP. The structure and function of microbiota in BALF were identified by metagenomic next-generation sequencing (mNGS). The community evenness of LRT microbiota within the IIM-ILD group was marginally lower compared to the other CTD-ILD and CAP groups. Nonetheless, there were no noticeable differences. The species community structure was similar among the three groups, based on the Bray-Curtis distance between the samples. At the level of genus, the IIM-ILD group displayed a considerably higher abundance of Pseudomonas and Corynebacterium in comparison to the CAP group (  
doi_str_mv 10.3389/fmed.2024.1338947
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_833a39d152264874961938e28a259f44</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_833a39d152264874961938e28a259f44</doaj_id><sourcerecordid>3041233095</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-bf345962f889f03cb95631940d41d13e601aa5a6fc5e4f77f3ef8d8871219c8e3</originalsourceid><addsrcrecordid>eNpVUs1u1DAYjBCIVqUPwAX5yGW3_ktinxCqgFYq4gISN-tL8nnXlRMvtrPVPgGvjdNdqvZke76ZseWZqnrP6FoIpa_siMOaUy7XbDnL9lV1zrluVqpWv18_259VlyndU0qZ4LVk4m11JlQjhKDNefX3u-tj6FwYkViEPEdMxE2ki2HqtwH8HoOHSDzsYVMofnYDCZbsIDucciIPLm-JG1woyNb1RWs9jCPkEA9kPDzCh1VEDxmHMs0YU3bZgSd-njZkcAkh4bvqjQWf8PK0XlS_vn75eX2zuvvx7fb6892ql0zlVWeFrHXDrVLaUtF3um4E05IOkg1MYEMZQA2N7WuUtm2tQKsGpVrGme4Viovq9ug7BLg3u-hGiAcTwJlHIMSNgZhd79EoIUDogdWcN1K1UjdMC4VcAa-1lbJ4fTp67eauhNGX_4jgX5i-nExuazZhbxijnLW8Lg4fTw4x_JkxZTO61KP3MGGYkxFUMl6C0guVHaklrpQi2qd7GDVLAcxSCLMUwpwKUTQfnj_wSfE_fvEPYQa1Ug</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3041233095</pqid></control><display><type>article</type><title>Microbiome features in bronchoalveolar lavage fluid of patients with idiopathic inflammatory myopathy-related interstitial lung disease</title><source>PubMed Central Free</source><creator>Zhang, Liyan ; Liu, Xueqing ; Fan, Bijun ; Chen, Jiajun ; Chen, Jie ; Li, Qiuhong ; Wu, Xueling</creator><creatorcontrib>Zhang, Liyan ; Liu, Xueqing ; Fan, Bijun ; Chen, Jiajun ; Chen, Jie ; Li, Qiuhong ; Wu, Xueling</creatorcontrib><description>Interstitial lung disease (ILD) is a common complication of idiopathic inflammatory myopathy (IIM), which is one of the connective tissue diseases (CTD). It can lead to poor prognosis and increased mortality. However, the distribution and role of the lower respiratory tract (LRT) microbiome in patients with IIM-ILD remains unclear. This study aimed to investigate the microbial diversity and community differences in bronchoalveolar lavage fluid (BALF) in patients with IIM-ILD. From 28 June 2021 to 26 December 2023, 51 individual BALF samples were enrolled, consisting of 20 patients with IIM-ILD, 16 patients with other CTD-ILD (including 8 patients with SLE and 8 with RA) and 15 patients with CAP. The structure and function of microbiota in BALF were identified by metagenomic next-generation sequencing (mNGS). The community evenness of LRT microbiota within the IIM-ILD group was marginally lower compared to the other CTD-ILD and CAP groups. Nonetheless, there were no noticeable differences. The species community structure was similar among the three groups, based on the Bray-Curtis distance between the samples. At the level of genus, the IIM-ILD group displayed a considerably higher abundance of Pseudomonas and Corynebacterium in comparison to the CAP group (  &lt; 0.01,  &lt; 0.05). At the species level, we found that the relative abundance of increased significantly in the IIM-ILD group compared to the CAP group (  &lt; 0.05). Additionally, the relative abundance of was significantly higher in other CTD-ILD groups compared to that in the IIM-ILD group (  &lt; 0.05). Of all the clinical indicators examined in the correlation analysis, ferritin level demonstrated the strongest association with LRT flora, followed by Serum interleukin-6 level (  &lt; 0.05). Our research has identified particular LRT microorganisms that were found to be altered in the IIM-ILD group and were significantly associated with immune function and inflammatory markers in patients. The lower respiratory tract microbiota has potential in the diagnosis and treatment of IIM-ILD.</description><identifier>ISSN: 2296-858X</identifier><identifier>EISSN: 2296-858X</identifier><identifier>DOI: 10.3389/fmed.2024.1338947</identifier><identifier>PMID: 38633306</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>idiopathic inflammatory myopathy ; interstitial lung disease ; lower respiratory tract ; Medicine ; metagenomic next-generation sequencing ; microbiome</subject><ispartof>Frontiers in medicine, 2024-04, Vol.11, p.1338947-1338947</ispartof><rights>Copyright © 2024 Zhang, Liu, Fan, Chen, Chen, Li and Wu.</rights><rights>Copyright © 2024 Zhang, Liu, Fan, Chen, Chen, Li and Wu. 2024 Zhang, Liu, Fan, Chen, Chen, Li and Wu</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c418t-bf345962f889f03cb95631940d41d13e601aa5a6fc5e4f77f3ef8d8871219c8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11021725/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11021725/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38633306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Liyan</creatorcontrib><creatorcontrib>Liu, Xueqing</creatorcontrib><creatorcontrib>Fan, Bijun</creatorcontrib><creatorcontrib>Chen, Jiajun</creatorcontrib><creatorcontrib>Chen, Jie</creatorcontrib><creatorcontrib>Li, Qiuhong</creatorcontrib><creatorcontrib>Wu, Xueling</creatorcontrib><title>Microbiome features in bronchoalveolar lavage fluid of patients with idiopathic inflammatory myopathy-related interstitial lung disease</title><title>Frontiers in medicine</title><addtitle>Front Med (Lausanne)</addtitle><description>Interstitial lung disease (ILD) is a common complication of idiopathic inflammatory myopathy (IIM), which is one of the connective tissue diseases (CTD). It can lead to poor prognosis and increased mortality. However, the distribution and role of the lower respiratory tract (LRT) microbiome in patients with IIM-ILD remains unclear. This study aimed to investigate the microbial diversity and community differences in bronchoalveolar lavage fluid (BALF) in patients with IIM-ILD. From 28 June 2021 to 26 December 2023, 51 individual BALF samples were enrolled, consisting of 20 patients with IIM-ILD, 16 patients with other CTD-ILD (including 8 patients with SLE and 8 with RA) and 15 patients with CAP. The structure and function of microbiota in BALF were identified by metagenomic next-generation sequencing (mNGS). The community evenness of LRT microbiota within the IIM-ILD group was marginally lower compared to the other CTD-ILD and CAP groups. Nonetheless, there were no noticeable differences. The species community structure was similar among the three groups, based on the Bray-Curtis distance between the samples. At the level of genus, the IIM-ILD group displayed a considerably higher abundance of Pseudomonas and Corynebacterium in comparison to the CAP group (  &lt; 0.01,  &lt; 0.05). At the species level, we found that the relative abundance of increased significantly in the IIM-ILD group compared to the CAP group (  &lt; 0.05). Additionally, the relative abundance of was significantly higher in other CTD-ILD groups compared to that in the IIM-ILD group (  &lt; 0.05). Of all the clinical indicators examined in the correlation analysis, ferritin level demonstrated the strongest association with LRT flora, followed by Serum interleukin-6 level (  &lt; 0.05). Our research has identified particular LRT microorganisms that were found to be altered in the IIM-ILD group and were significantly associated with immune function and inflammatory markers in patients. The lower respiratory tract microbiota has potential in the diagnosis and treatment of IIM-ILD.</description><subject>idiopathic inflammatory myopathy</subject><subject>interstitial lung disease</subject><subject>lower respiratory tract</subject><subject>Medicine</subject><subject>metagenomic next-generation sequencing</subject><subject>microbiome</subject><issn>2296-858X</issn><issn>2296-858X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVUs1u1DAYjBCIVqUPwAX5yGW3_ktinxCqgFYq4gISN-tL8nnXlRMvtrPVPgGvjdNdqvZke76ZseWZqnrP6FoIpa_siMOaUy7XbDnL9lV1zrluVqpWv18_259VlyndU0qZ4LVk4m11JlQjhKDNefX3u-tj6FwYkViEPEdMxE2ki2HqtwH8HoOHSDzsYVMofnYDCZbsIDucciIPLm-JG1woyNb1RWs9jCPkEA9kPDzCh1VEDxmHMs0YU3bZgSd-njZkcAkh4bvqjQWf8PK0XlS_vn75eX2zuvvx7fb6892ql0zlVWeFrHXDrVLaUtF3um4E05IOkg1MYEMZQA2N7WuUtm2tQKsGpVrGme4Viovq9ug7BLg3u-hGiAcTwJlHIMSNgZhd79EoIUDogdWcN1K1UjdMC4VcAa-1lbJ4fTp67eauhNGX_4jgX5i-nExuazZhbxijnLW8Lg4fTw4x_JkxZTO61KP3MGGYkxFUMl6C0guVHaklrpQi2qd7GDVLAcxSCLMUwpwKUTQfnj_wSfE_fvEPYQa1Ug</recordid><startdate>20240403</startdate><enddate>20240403</enddate><creator>Zhang, Liyan</creator><creator>Liu, Xueqing</creator><creator>Fan, Bijun</creator><creator>Chen, Jiajun</creator><creator>Chen, Jie</creator><creator>Li, Qiuhong</creator><creator>Wu, Xueling</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20240403</creationdate><title>Microbiome features in bronchoalveolar lavage fluid of patients with idiopathic inflammatory myopathy-related interstitial lung disease</title><author>Zhang, Liyan ; Liu, Xueqing ; Fan, Bijun ; Chen, Jiajun ; Chen, Jie ; Li, Qiuhong ; Wu, Xueling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-bf345962f889f03cb95631940d41d13e601aa5a6fc5e4f77f3ef8d8871219c8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>idiopathic inflammatory myopathy</topic><topic>interstitial lung disease</topic><topic>lower respiratory tract</topic><topic>Medicine</topic><topic>metagenomic next-generation sequencing</topic><topic>microbiome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Liyan</creatorcontrib><creatorcontrib>Liu, Xueqing</creatorcontrib><creatorcontrib>Fan, Bijun</creatorcontrib><creatorcontrib>Chen, Jiajun</creatorcontrib><creatorcontrib>Chen, Jie</creatorcontrib><creatorcontrib>Li, Qiuhong</creatorcontrib><creatorcontrib>Wu, Xueling</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Frontiers in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Liyan</au><au>Liu, Xueqing</au><au>Fan, Bijun</au><au>Chen, Jiajun</au><au>Chen, Jie</au><au>Li, Qiuhong</au><au>Wu, Xueling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbiome features in bronchoalveolar lavage fluid of patients with idiopathic inflammatory myopathy-related interstitial lung disease</atitle><jtitle>Frontiers in medicine</jtitle><addtitle>Front Med (Lausanne)</addtitle><date>2024-04-03</date><risdate>2024</risdate><volume>11</volume><spage>1338947</spage><epage>1338947</epage><pages>1338947-1338947</pages><issn>2296-858X</issn><eissn>2296-858X</eissn><abstract>Interstitial lung disease (ILD) is a common complication of idiopathic inflammatory myopathy (IIM), which is one of the connective tissue diseases (CTD). It can lead to poor prognosis and increased mortality. However, the distribution and role of the lower respiratory tract (LRT) microbiome in patients with IIM-ILD remains unclear. This study aimed to investigate the microbial diversity and community differences in bronchoalveolar lavage fluid (BALF) in patients with IIM-ILD. From 28 June 2021 to 26 December 2023, 51 individual BALF samples were enrolled, consisting of 20 patients with IIM-ILD, 16 patients with other CTD-ILD (including 8 patients with SLE and 8 with RA) and 15 patients with CAP. The structure and function of microbiota in BALF were identified by metagenomic next-generation sequencing (mNGS). The community evenness of LRT microbiota within the IIM-ILD group was marginally lower compared to the other CTD-ILD and CAP groups. Nonetheless, there were no noticeable differences. The species community structure was similar among the three groups, based on the Bray-Curtis distance between the samples. At the level of genus, the IIM-ILD group displayed a considerably higher abundance of Pseudomonas and Corynebacterium in comparison to the CAP group (  &lt; 0.01,  &lt; 0.05). At the species level, we found that the relative abundance of increased significantly in the IIM-ILD group compared to the CAP group (  &lt; 0.05). Additionally, the relative abundance of was significantly higher in other CTD-ILD groups compared to that in the IIM-ILD group (  &lt; 0.05). Of all the clinical indicators examined in the correlation analysis, ferritin level demonstrated the strongest association with LRT flora, followed by Serum interleukin-6 level (  &lt; 0.05). Our research has identified particular LRT microorganisms that were found to be altered in the IIM-ILD group and were significantly associated with immune function and inflammatory markers in patients. The lower respiratory tract microbiota has potential in the diagnosis and treatment of IIM-ILD.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>38633306</pmid><doi>10.3389/fmed.2024.1338947</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2296-858X
ispartof Frontiers in medicine, 2024-04, Vol.11, p.1338947-1338947
issn 2296-858X
2296-858X
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_833a39d152264874961938e28a259f44
source PubMed Central Free
subjects idiopathic inflammatory myopathy
interstitial lung disease
lower respiratory tract
Medicine
metagenomic next-generation sequencing
microbiome
title Microbiome features in bronchoalveolar lavage fluid of patients with idiopathic inflammatory myopathy-related interstitial lung disease
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A07%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microbiome%20features%20in%20bronchoalveolar%20lavage%20fluid%20of%20patients%20with%20idiopathic%20inflammatory%20myopathy-related%20interstitial%20lung%20disease&rft.jtitle=Frontiers%20in%20medicine&rft.au=Zhang,%20Liyan&rft.date=2024-04-03&rft.volume=11&rft.spage=1338947&rft.epage=1338947&rft.pages=1338947-1338947&rft.issn=2296-858X&rft.eissn=2296-858X&rft_id=info:doi/10.3389/fmed.2024.1338947&rft_dat=%3Cproquest_doaj_%3E3041233095%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c418t-bf345962f889f03cb95631940d41d13e601aa5a6fc5e4f77f3ef8d8871219c8e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3041233095&rft_id=info:pmid/38633306&rfr_iscdi=true