Optimization of the Microwave-Assisted Extraction Process of Bioactive Compounds from Annatto Seeds ( Bixa orellana L.)

This study deals with the extraction, optimization, and evaluation of the antioxidant and antimicrobial activities of bioactive compounds obtained from the seeds of annatto using microwave-assisted extraction as compared to leaching. Annatto seeds were subjected to a microwave treatment of 2450 MHz...

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Bibliographic Details
Published in:Antioxidants 2019-02, Vol.8 (2), p.37
Main Authors: Quiroz, Julian Q, Torres, Angélica C, Ramirez, Luisa M, Garcia, Mariluz S, Gomez, Gelmy C, Rojas, John
Format: Article
Language:English
Subjects:
Antimicrobial activity
Antimicrobial agents
antioxidant activity
Antioxidants
Bioactive compounds
bixin
Caustic soda
Ethanol
Inflammation
Leaching
Methods
Microwave radiation
polyphenol compounds
Polyphenols
Seeds
Sodium
Solvents
Statistical analysis
Variance analysis
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Summary:This study deals with the extraction, optimization, and evaluation of the antioxidant and antimicrobial activities of bioactive compounds obtained from the seeds of annatto using microwave-assisted extraction as compared to leaching. Annatto seeds were subjected to a microwave treatment of 2450 MHz and power of 700 watts using a response surface design involving four factors: pH (4⁻11), solvent concentration (ethanol) (50⁻96 %), solvent-to-seed ratio (2⁻10), and microwave exposure time (0⁻5 min). The contents of polyphenol compounds and bixin were taken as response variables. Subsequently, the antioxidant and antimicrobial activities were assessed at the optimal processing conditions predicted by the experimental design. Microwaves, solvent concentration, and the solvent-to-seed ratio showed a statistically significant effect for the extraction of polyphenol compounds and bixin. Thus, microwaves accelerated the extraction of those compounds and the slight increase in temperature caused some degradation of the polyphenol compounds. The microwave-assisted extraction increased the contents of polyphenols and bixin along with their antioxidant activity as compared to leaching extraction. However, this technique does not significantly improve the antimicrobial activity against and .
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox8020037