Cilostazol Ameliorates Motor Dysfunction and Schwann Cell Impairment in Streptozotocin-Induced Diabetic Rats

This study investigated the effects of cilostazol on motor dysfunction, spinal motor neuron abnormalities, and schwannopathy in rats with diabetes. Diabetes mellitus (DM) was induced in rats via femoral intravenous streptozotocin (STZ) injection (60 mg/kg). After successful DM induction, cilostazol...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-07, Vol.25 (14), p.7847
Main Authors: Chang, Lin-Li, Wu, Yu-Ming, Wang, Hung-Chen, Tseng, Kuang-Yi, Wang, Yi-Hsuan, Lu, Yen-Mou, Cheng, Kuang-I
Format: Article
Language:English
Subjects:
Amputation
Cholesterol
cholinergic neuron
cilostazol
Diabetes
Diabetic neuropathy
diabetic rat
Hyperalgesia
Hyperglycemia
Ischemia
motor neuron
Nervous system
neuropathy
Pathogenesis
Peripheral neuropathy
Pharmaceutical industry
Running
schwannopathy
Triglycerides
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Summary:This study investigated the effects of cilostazol on motor dysfunction, spinal motor neuron abnormalities, and schwannopathy in rats with diabetes. Diabetes mellitus (DM) was induced in rats via femoral intravenous streptozotocin (STZ) injection (60 mg/kg). After successful DM induction, cilostazol was administered on day 15 via oral gavage (100 mg/kg/day) for 6 weeks until sacrifice. Behavioral assays, including motor function, were performed weekly. The sciatic nerve, L5 spinal cord, and spinal ventral root were collected to evaluate the expression of the glial fibrillary acidic protein (GFAP), myelin protein zero (P0), and choline acetyltransferase (ChAT) by immunofluorescence and Western blotting. DM rats displayed decreased running speeds, running distances, and toe spread but increased foot pressure. In addition, loss of non-myelinating Schwann cells and myelin sheaths was observed in the sciatic nerve and L5 spinal ventral root. Reduced numbers of motor neurons were also found in the L5 spinal ventral horn. Cilostazol administration significantly potentiated running speed and distance; increased hind paw toe spread; and decreased foot pressure. In the sciatic nerve and L5 spinal ventral root, cilostazol treatment significantly improved non-myelinated Schwann cells and increased myelin mass. ChAT expression in motor neurons in the spinal ventral horn was improved, but not significantly. Cilostazol administration may protect sensorimotor function in diabetic rats.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25147847