Biological Characterization and Metabolic Variations among Cell-Free Supernatants Produced by Selected Plant-Based Lactic Acid Bacteria

• Published in: Metabolites 2023-07, Vol.13 (7), p.849
• Main Authors: Qadi, Wasim S M, Mediani, Ahmed, Kasim, Zalifah Mohd, Misnan, Norazlan Mohmad, Sani, Norrakiah Abdullah, Jamar, Nur Hidayah
• Format: Article
• Language: English
• Subjects: 1H NMR; Acetic acid; Acids; Analysis; antibacterial; Antibacterial activity; Antibiotics; Antimicrobial agents; antioxidant; Antioxidants; Bacteria; Drug resistance; E coli; Endopeptidase K; Fermentation; Foodborne pathogens; Functional foods & nutraceuticals; Histidine; Lactic acid; Lactic acid bacteria; Metabolites; metabolomics; Microorganisms; Minimum inhibitory concentration; NMR; Nuclear magnetic resonance; Pathogens; Pepsin; Principal components analysis; probiotic; Probiotics; Properties; Propylene glycol; Salmonella; Spectroscopy; Spectrum analysis; Strains (organisms); Uracil; Uridine; γ-Aminobutyric acid
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo13070849

The aim of this research was to assess the antibacterial and antioxidant properties as well as the variation in metabolites of the cell-free supernatant (CFS) produced by lactic acid bacteria (LAB) from local plants: ngue16, ng10, w3, and w6. The tested strains exhibited inhibitory effects against pathogens, including , , , , Typhimurium, using the agar spot assay and well diffusion method. The CFS from all four strains displayed antibacterial activity against these pathogens with minimum inhibitory concentration (MIC) values ranging from 3.12 to 12.5 mg/mL and minimal bactericidal concentration (MBC) values ranging from 6.25 to 25.0 mg/mL. Moreover, the CFS demonstrated resilience within specific pH (3-8) and temperature (60-100 °C) ranges and lost its activity when treated with enzymes, such as Proteinase K and pepsin. Furthermore, the CFS exhibited antioxidant properties as evidenced by their ability to inhibit the formation of two radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) compared to the negative control, De Man, Rogosa, and Sharpe (MRS) broth. The use of proton-based nuclear magnetic resonance ( H-NMR) spectroscopy revealed the presence and quantification of 48 metabolites in both the CFS and MRS broths. Principal Component Analysis (PCA) effectively differentiated between CFS and MRS broth by identifying the specific metabolites responsible for the observed differences. The partial least squares (PLS) model demonstrated a significant correlation between the metabolites in the LAB supernatant and the tested antibacterial and antioxidant activities. Notably, anserine, GABA, acetic acid, lactic acid, uracil, uridine, propylene glycol, isopropanol, serine, histidine, and indol-3-lactate were identified as the compounds contributing the most to the highest antibacterial and antioxidant activities in the supernatant. These findings suggest that the LAB strains investigated have the potential to be utilized in the production of functional foods and the development of pharmaceutical products.