Curcumin-based-fluorescent probes targeting ALDH1A3 as a promising tool for glioblastoma precision surgery and early diagnosis

Glioblastoma (GBM) is the most aggressive primary brain tumour for which both effective treatments and efficient tools for an early-stage diagnosis are lacking. Herein, we present curcumin-based fluorescent probes that are able to bind to aldehyde dehydrogenase 1A3 (ALDH1A3), an enzyme overexpressed...

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Published in:Communications biology 2022-09, Vol.5 (1), p.895-895, Article 895
Main Authors: Gelardi, Edoardo L. M., Caprioglio, Diego, Colombo, Giorgia, Del Grosso, Erika, Mazzoletti, Daniele, Mattoteia, Daiana, Salamone, Stefano, Ferraris, Davide M., Aronica, Eleonora, Nato, Giulia, Buffo, Annalisa, Rizzi, Menico, Magrassi, Lorenzo, Minassi, Alberto, Garavaglia, Silvia
Format: Article
Language:English
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Aldehyde dehydrogenase
Aldehydes
Biology
Biomedical and Life Sciences
Brain cancer
Brain tumors
Curcumin
Dehydrogenases
Diagnosis
Enzymes
Fluorescent indicators
Glioblastoma
Glioblastoma cells
Glioma cells
Invasiveness
Isoenzymes
Life Sciences
Neurosurgery
Stem cells
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Summary:Glioblastoma (GBM) is the most aggressive primary brain tumour for which both effective treatments and efficient tools for an early-stage diagnosis are lacking. Herein, we present curcumin-based fluorescent probes that are able to bind to aldehyde dehydrogenase 1A3 (ALDH1A3), an enzyme overexpressed in glioma stem cells (GSCs) and associated with stemness and invasiveness of GBM. Two compounds are selective versus ALDH1A3, without showing any appreciable interaction with other ALDH1A isoenzymes. Indeed, their fluorescent signal is detectable only in our positive controls in vitro and absent in cells that lack ALDH1A3. Remarkably, in vivo, our Probe selectively accumulate in glioblastoma cells, allowing the identification of the growing tumour mass. The significant specificity of our compounds is the necessary premise for their further development into glioblastoma cells detecting probes to be possibly used during neurosurgical operations. Curcumin-based fluorescent probes are presented that are able to selectively bind to aldehyde dehydrogenase 1A3 (ALDH1A3), an enzyme overexpressed in glioma stem cells and specifically identify glioblastoma cells in vitro and in vivo.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-022-03834-7