Levels of DNA cytosine methylation in the Drosophila genome

Insects provide an accessible system to study the contribution of DNA methylation to complex epigenetic phenotypes created to regulate gene expression, chromatin states, imprinting and dosage compensation. The members of genus have been used as a model system to study aspects of biology like develop...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2018-07, Vol.6, p.e5119-e5119, Article e5119
Main Authors: Deshmukh, Saniya, Ponnaluri, Vk Chaithanya, Dai, Nan, Pradhan, Sriharsa, Deobagkar, Deepti
Format: Article
Language:English
Subjects:
5-methylcytosine
Biochemistry
Chromatin
Chromatography
Cytosine
Deoxyribonucleic acid
DNA
DNA methylation
Dosage compensation
Drosophila
Drosophila melanogaster
Embryos
Epigenetics
Females
Gene expression
Genetic aspects
Genetic testing
Genomes
Imprinting
Insects
Laboratories
Life cycles
Liquid chromatography
Mammals
Mass spectrometry
Mass spectroscopy
Methylation
Molecular Biology
Mus musculus
Phenotypes
Phylogeny
Scientific imaging
UHPLC-QQQ
Zoology
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Summary:Insects provide an accessible system to study the contribution of DNA methylation to complex epigenetic phenotypes created to regulate gene expression, chromatin states, imprinting and dosage compensation. The members of genus have been used as a model system to study aspects of biology like development, behaviour and genetics. Despite the popularity of as a genetic and epigenetic model organism, DNA methylation studies are limited due to low levels of genomic 5-methylcytosine. Our study employs a sensitive liquid chromatography-mass spectrometry (LCMS) based method to quantify the levels of 5-methylcytosine from the genomic DNA in different members of the genus . Our results reveal that, despite being phylogenetically related, there is a marked variation in the levels of 5-methylcytosine between the genomes of the members of genus . Also, there is a change in the genomic levels of 5-methylcytosine through each life cycle stage of holometabolous development in .
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.5119