Quo Vadis Caenorhabditis elegans Metabolomics-A Review of Current Methods and Applications to Explore Metabolism in the Nematode

Metabolomics and lipidomics recently gained interest in the model organism ( ). The fast development, easy cultivation and existing forward and reverse genetic tools make the small nematode an ideal organism for metabolic investigations in development, aging, different disease models, infection, or...

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Bibliographic Details
Published in:Metabolites 2021-04, Vol.11 (5), p.284
Main Authors: Salzer, Liesa, Witting, Michael
Format: Article
Language:English
Subjects:
Aging
Caenorhabditis elegans
Lipid metabolism
lipidomics
Lipids
Liquid chromatography
Mass spectroscopy
mass-spectrometry
Metabolites
Metabolomics
NMR
Nuclear magnetic resonance
Review
Reviews
Secondary metabolites
Worms
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Summary:Metabolomics and lipidomics recently gained interest in the model organism ( ). The fast development, easy cultivation and existing forward and reverse genetic tools make the small nematode an ideal organism for metabolic investigations in development, aging, different disease models, infection, or toxicology research. The conducted type of analysis is strongly depending on the biological question and requires different analytical approaches. Metabolomic analyses in have been performed using nuclear magnetic resonance (NMR) spectroscopy, direct infusion mass spectrometry (DI-MS), gas-chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) or combinations of them. In this review we provide general information on the employed techniques and their advantages and disadvantages in regard to metabolomics. Additionally, we reviewed different fields of application, e.g., longevity, starvation, aging, development or metabolism of secondary metabolites such as ascarosides or maradolipids. We also summarised applied bioinformatic tools that recently have been used for the evaluation of metabolomics or lipidomics data from . Lastly, we curated metabolites and lipids from the reviewed literature, enabling a prototypic collection which serves as basis for a future specific metabolome database.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo11050284