The miR-20a/miR-92b Profile Is Associated with Circulating γδ T-Cell Perturbations in Mild Psoriasis

• Published in: International journal of molecular sciences 2023-02, Vol.24 (5), p.4323
• Main Authors: Tokić, Stana, Jirouš, Maja, Plužarić, Vera, Mihalj, Martina, Šola, Marija, Tolušić Levak, Maja, Glavaš, Kristina, Balogh, Peter, Štefanić, Mario
• Format: Article
• Language: English
• Subjects: Analysis; Cell adhesion; Cell adhesion molecules; Cells; Communication; Composition; Cytokine receptors; Cytomegalovirus; Cytotoxicity; DNA-binding protein; Epigenetics; Etiology; hsa-let-7c; hsa-mir-20a; hsa-mir-29a; hsa-mir-92b; Humans; Infections; Intraepithelial Lymphocytes - metabolism; Lymphocytes; Lymphocytes T; MicroRNAs; MicroRNAs - metabolism; miRNA; Psoriasis; Psoriasis - metabolism; Psoriasis vulgaris; Receptors, Antigen, T-Cell, gamma-delta - metabolism; RNA; T cells; T-Lymphocyte Subsets - metabolism; γδ T cells
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24054323

Psoriasis vulgaris (PV) is an autoinflammatory dermatosis of unknown etiology. Current evidence suggests a pathogenic role of γδT cells, but the growing complexity of this population has made the offending subset difficult to pinpoint. The work on γδTCR and γδTCR subsets, which express intermediate and high levels of γδTCR at their surface, respectively, is particularly scarce, leaving their inner workings in PV essentially unresolved. We have shown here that the γδTCR /γδTCR cell composition and their transcriptom are related to the differential miRNA expression by performing a targeted miRNA and mRNA quantification (RT-qPCR) in multiplexed, flow-sorted γδ blood T cells from healthy controls ( = 14) and patients with PV ( = 13). A significant loss of miR-20a in bulk γδT cells (~fourfold decrease, PV vs. controls) largely mirrored increasing Vδ1 Vδ2 and γδ Vδ1 Vδ2 cell densities in the bloodstream, culminating in a relative excess of γδ Vδ1 Vδ2 cells for PV. Transcripts encoding DNA-binding factors ( ), cytokine receptors ( ), and cell adhesion molecules ( ) were depleted in the process, closely tracking miR-20a availability in bulk γδ T-cell RNA. Compared to controls, PV was also associated with enhanced miR-92b expression (~13-fold) in bulk γδT cells that lacked association with the γδT cell composition. The miR-29a and let-7c expressions remained unaltered in case-control comparisons. Overall, our data expand the current landscape of the peripheral γδT cell composition, underlining changes in its mRNA/miRNA transcriptional circuits that may inform PV pathogenesis.