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Cyclic Tetrapyrrolic Photosensitisers from the leaves of Phaeanthus ophthalmicus
Background Twenty-seven extracts from 26 plants were identified as photo-cytotoxic in the course of our bioassay guided screening program for photosensitisers from 128 extracts prepared from 64 terrestrial plants in two different collection sites in Malaysia - Royal Belum Forest Reserve in the State...
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Published in: | Chemistry Central journal 2011-06, Vol.5 (1), p.32-32, Article 32 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Background
Twenty-seven extracts from 26 plants were identified as photo-cytotoxic in the course of our bioassay guided screening program for photosensitisers from 128 extracts prepared from 64 terrestrial plants in two different collection sites in Malaysia - Royal Belum Forest Reserve in the State of Perak and Gunung Nuang in the State of Selangor. One of the photo-cytotoxic extracts from the leaves of
Phaeanthus ophtalmicus
was further investigated.
Results
The ethanolic extract of the leaves from
Phaeanthus ophtalmicus
was able to reduce the
in vitro
viability of leukaemic HL60 cells to < 50% when exposed to 9.6 J/cm
2
of a broad spectrum light at a concentration of 20 μg/mL. Dereplication of the photo-cytotoxic fractions from
P. ophthalmicus
extracts based on TLC R
f
values and HPLC co-injection of reference tetrapyrrolic compounds enabled quick identification of known photosensitisers, pheophorbide-
a
, pheophorbide-
a
methyl ester, 13
2
-hydroxypheophorbide-
a
methyl ester, pheophytin-
a
and 15
1
-hydroxypurpurin 7-lactone dimethyl ester. In addition, compound
1
which was not previously isolated as a natural product was also identified as 7-formyl-15
1
-hydroxypurpurin-7-lactone methyl ester using standard spectroscopic techniques.
Conclusions
Our results suggest that the main photosensitisers in plants are based on the cyclic tetrapyrrole structure and photosensitisers with other structures, if present, are present in very minor amounts or are not as active as those with the cyclic tetrapyrrole structure. |
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ISSN: | 1752-153X 1752-153X |
DOI: | 10.1186/1752-153X-5-32 |