In vitro supplementation with the porcine plasma product, betaGRO®, stimulates activity of porcine fetal myoblasts and neonatal satellite cells in a divergent manner

• Published in: Animal (Cambridge, England) England), 2018-09, Vol.12 (9), p.1912-1920
• Main Authors: Vaughn, M. A., Phelps, K. J., Gonzalez, J. M.
• Format: Article
• Language: English
• Subjects: Animals; betaGRO; Cell culture; Cell Differentiation; Cell proliferation; Cells (biology); Cells, Cultured; Culture media; Dietary fiber; differentiation; Experiments; Fetus; Fetuses; Gestation; Hogs; Insulin; Insulin-like growth factors; Kinases; Laboratories; Muscle Fibers, Skeletal; Myoblasts; Myoblasts - drug effects; Myoblasts - physiology; Myogenesis; Myotubes; Neonates; Phosphorylation; Physiology and functional biology; Plasma; porcine fetal myoblasts; porcine satellite cells; proliferation; Protein synthesis; Proteins; Research Article; Satellite cells; Satellite Cells, Skeletal Muscle - physiology; Supplements; Swine - physiology
• ISSN: 1751-7311; 1751-732X
• DOI: 10.1017/S1751731117003329

Two separate experiments were conducted to evaluate the effect of betaGRO® supplementation on in vitro porcine fetal myoblasts (PFM) and porcine satellite cells (PSC) proliferation, fusion and myotube thickness. The PFM and PSC were isolated from the m. longissimus dorsi of day 60 of gestation fetuses and piglets within 24 h of birth, respectively. Proliferation assays were conducted as 4×3 factorial arrangements with time of culture (24, 48, 72, 96 h) and media treatment (standard porcine media supplemented with 10% (vol/vol) fetal bovine serum (HS); HS without 10% fetal bovine serum (LS); and LS supplemented with 10 mg/ml betaGRO® (BG)) as main effects. Fusion and myotube growth assays were conducted as 2×2 factorial designs with serum concentration (HS or LS), and betaGRO® inclusion (0 or 10 mg/ml) as main effects. There was a treatment×time interaction and betaGRO®×serum interactions for proliferation, fusion and myotube thickness of PFM (P