Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes

Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising m...

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Bibliographic Details
Published in:International journal of molecular sciences 2017-08, Vol.18 (8), p.1691
Main Authors: Oliinyk, Olena S, Chernov, Konstantin G, Verkhusha, Vladislav V
Format: Article
Language:English
Subjects:
allophycocyanin
Bacteria
Bacteria - chemistry
Bacteria - genetics
Bacteria - metabolism
bacterial photoreceptor
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biosensing Techniques - methods
Biosensors
cyanobacteriochrome
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Fluorescent indicators
I.R. radiation
Infrared Rays
Infrared spectra
Mammalian cells
Metabolism
Molecular evolution
Near infrared radiation
near-infrared fluorescent protein
Photoreceptors
Phycocyanin - chemistry
Phycocyanin - genetics
Phycocyanin - metabolism
phytochrome
Phytochrome - chemistry
Phytochrome - genetics
Phytochrome - metabolism
Phytochromes
Proteins
Review
tetrapyrrole
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Summary:Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising molecular templates to design NIR fluorescent proteins (FPs) and biosensors for studies in mammalian cells and whole animals. Here, we review structures, photochemical properties and molecular functions of several families of bacterial photoreceptors. We next analyze molecular evolution approaches to develop NIR FPs and biosensors. We then discuss phenotypes of current BphP-based NIR FPs and compare them with FPs derived from CBCRs and APCs. Lastly, we overview imaging applications of NIR FPs in live cells and in vivo. Our review provides guidelines for selection of existing NIR FPs, as well as engineering approaches to develop NIR FPs from the novel natural templates such as CBCRs.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms18081691