Determination of the contents of A- and B-starches in barley using low angle laser light scattering

A method was proposed for the determination of the contents of A- and B-starches in barley and for the use in plant breeding stations and brewing/malting laboratories. A combination was used of classical methods (incl. crushing of barley kernels by a roll crusher, steeping in 0.02M HCl, repeated rub...

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Bibliographic Details
Published in:Czech Journal of Food Sciences 2006-01, Vol.24 (1), p.11-18
Main Authors: Bohacenko, I.(Vyzkumny Ustav Potravinarsky, Prague (Czech Republic)) E-mail:i.bohacenko@vupp.cz, Chmelik, J.(Akademie Ved, Brno (Czech Republic). Ustav Analyticke Chemie), Psota, V.(Vyzkumny Ustav Pivovarsky a Sladarsky, Brno (Czech Republic))
Format: Article
Language:English
Subjects:
a-starch
ALMIDON
AMIDON
ANALYTICAL METHODS
b-starch
BARLEY
Biological materials
Breweries
Brewing
CEBADA
Cellulase
Centrifugation
CHEMICAL COMPOSITION
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Confidence intervals
DIMENSION
DIMENSIONS
HORDEUM VULGARE
Kernels
Light scattering
low angle laser light scattering
ORGE
Particle size distribution
Plant breeding
Roll crushers
Rubbing
Size distribution
STARCH
starch granules
Starches
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
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Summary:A method was proposed for the determination of the contents of A- and B-starches in barley and for the use in plant breeding stations and brewing/malting laboratories. A combination was used of classical methods (incl. crushing of barley kernels by a roll crusher, steeping in 0.02M HCl, repeated rubbing and filtering through the sieve of 0.08 mm), and novel pieces of know-how (treatment with beta-glucanase and cellulase, alkalisation at pH=10 and centrifugation of crude starch suspension through the layer of CsCl). In this way, barley starch of high purity was obtained at a very low loss, using Low Angle Laser Light Scattering for the determination of the size distribution of starch granules. The boundary of the particle size between the peaks of A-starch and B-starch (7 microm) was evaluated from the distribution curves, while the contents of A- and B-starches were calculated from the cumulative curves. The method was validated, standard deviation and confidence interval were calculated.
ISSN:1212-1800
1805-9317
DOI:10.17221/3288-cjfs