Co-substrate model development and validation on pure sugars and corncob hemicellulosic hydrolysate for xylitol production

• Published in: Scientific reports 2024-10, Vol.14 (1), p.25928-13, Article 25928
• Main Authors: Feng, Juan, Techapun, Charin, Phimolsiripol, Yuthana, Rachtanapun, Pornchai, Phongthai, Suphat, Khemacheewakul, Julaluk, Taesuwan, Siraphat, Porninta, Kritsadaporn, Htike, Su Lwin, Mahakuntha, Chatchadaporn, Sommanee, Sumeth, Nunta, Rojarej, Kumar, Anbarasu, Leksawasdi, Noppol
• Format: Article
• Language: English
• Subjects: 631/326; 631/61; 639/705; Agricultural wastes; Batch culture; Biomass; Candida tropicalis; Candida tropicalis - growth & development; Candida tropicalis - metabolism; Co-substrate cultivation; Fermentation; Glucose; Glucose - metabolism; Hemicellulosic hydrolysate; Humanities and Social Sciences; Hydrolysates; Hydrolysis; Industrial wastes; Kinetics; Model development; Models, Biological; multidisciplinary; Polysaccharides - chemistry; Polysaccharides - metabolism; Resource management; Science; Science (multidisciplinary); Substrates; Sugarcane; Xylitol; Xylitol - metabolism; Xylitol production; Xylose; Xylose - chemistry; Xylose - metabolism; Yeast; Zea mays - chemistry; Zea mays - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-77462-y

A co-substrate model of Candida tropicalis TISTR 5306 cultivated in 10 - 100 g/L xylose and 1 - 10 g/L glucose at the ratio of 10:1 was developed based in part on modified Monod equation. The kinetic parameters include substrate limitation as well as substrate and product inhibitions with inclusion of threshold values. A general good fitting with average RSS total , R 2 , and MS total values of 162, 0.979, and 10.8, respectively, was achieved between ten simulated profiles and experimental kinetics data. The implementation of developed model on xylitol production from non-detoxified corncob hemicellulosic hydrolysate resulted in relatively good agreement with RSS total , R 2 , and MS total values of 368, 0.988, and 24.5, respectively. The developed model can be applied to predict microbial behavior in batch xylitol production system using hemicellulosic hydrolysate over a xylose range of 10 - 100 g/L and provide useful information for subsequent design of fed-batch and continuous systems to achieve the efficient sustainable resource management of this agricultural and agro-industrial waste.