Nitrogen Deficiency Enhances Eggplant Defense against Western Flower Thrips via the Induction of the Jasmonate Pathway

• Published in: Plants (Basel) 2024-01, Vol.13 (2), p.273
• Main Authors: Zheng, Yueqin, Liu, Qianxia, Shi, Shuang, Zhu, Xiaowen, Chen, Yong, Lin, Shuo, Tian, Houjun, Huang, Lanyan, Wei, Hui
• Format: Article
• Language: English
• Subjects: Amino acids; anti-herbivore resistance; Botanical research; Cellular signal transduction; Defense; Diseases and pests; Eggplant; Females; Flowers; Flowers & plants; Frankliniella occidentalis; Genes; Genomes; Herbivores; induced defense; Insects; Isoleucine; Jasmonates; Jasmonic acid; Kinases; Leaves; Methyl jasmonate; N deficiency; Nitrates; Nitrogen; Nitrogen in the body; Peroxidase; Pest resistance; Phenylalanine; Physiological aspects; Plant nutrition; Polyphenol oxidase; Rice; Seeds; Solanum melongena; Thrips; Valine; western flower thrips
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13020273

Plant nutrition is connected to defense against insect herbivores, but the exact mechanism underlying the effect of the nitrogen (N) supply on the anti-herbivore capacity of eggplants ( ) has not been studied in detail. Therefore, we examined the impact of low (LN, 0.5 mM) and high (HN, 5 mM) nitrate levels on eggplant resistance against the western flower thrips (WFT), a major destructive eggplant pest. Our results showed that LN plants displayed enhanced defense responses to WFT compared to HN plants. This included increased transcript levels of key genes in the jasmonic acid (JA) pathway, the accumulation of JA-amido conjugates (jasmonoyl-isoleucine, jasmonoyl-phenylalanine, and jasmonoyl-valine), JA precursor (12-oxophytodienoic acid), and methyl jasmonate, higher transcript levels of defense marker genes ( , , and ), and increased activities of polyphenol oxidase and peroxidase upon a WFT attack. Our findings suggest that N deficiency can prime JA-mediated defense responses in eggplants, resulting in increased anti-herbivore resistance.