Effect of Sample Transportation on the Proteome of Human Circulating Blood Extracellular Vesicles

• Published in: International journal of molecular sciences 2022-04, Vol.23 (9), p.4515
• Main Authors: Uldry, Anne-Christine, Maciel-Dominguez, Anabel, Jornod, Maïwenn, Buchs, Natasha, Braga-Lagache, Sophie, Brodard, Justine, Jankovic, Jovana, Bonadies, Nicolas, Heller, Manfred
• Format: Article
• Language: English
• Subjects: acceleration forces; Biological effects; Biological properties; Blood; Blood circulation; Blood Coagulation; Blood platelets; Blood Platelets - metabolism; Blood Specimen Collection - methods; circulating extracellular vesicles; clinical blood samples; Coagulation; Composition; Correlation analysis; Cytoskeleton; Extracellular Vesicles; Humans; Influence; label-free proteomics; Laboratories; Lymphocytes; Mass spectrometry; Microscopy; Mitochondria; Monocytes; Nanoparticles; Oscillations; Particle size; Particle size distribution; Plasma; pneumatical tube system transport; Protein composition; Protein interaction; Protein transport; Proteins; Proteome - metabolism; Proteomes; Proteomics; Scientific imaging; Size distribution; Tumors; Vesicles; vibration; Vibration analysis
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23094515

Circulating extracellular vesicles (cEV) are released by many kinds of cells and play an important role in cellular communication, signaling, inflammation modulation, coagulation, and tumor growth. cEV are of growing interest, not only as biomarkers, but also as potential treatment targets. However, very little is known about the effect of transporting biological samples from the clinical ward to the diagnostic laboratory, notably on the protein composition. Pneumatic tube systems (PTS) and human carriers (C) are both routinely used for transport, subjecting the samples to different ranges of mechanical forces. We therefore investigated qualitatively and quantitatively the effect of transport by C and PTS on the human cEV proteome and particle size distribution. We found that samples transported by PTS were subjected to intense, irregular, and multidirectional shocks, while those that were transported by C mostly underwent oscillations at a ground frequency of approximately 4 Hz. PTS resulted in the broadening of nanoparticle size distribution in platelet-free (PFP) but not in platelet-poor plasma (PPP). Cell-type specific cEV-associated protein abundances remained largely unaffected by the transport type. Since residual material of lymphocytes, monocytes, and platelets seemed to dominate cEV proteomes in PPP, it was concluded that PFP should be preferred for any further analyses. Differential expression showed that the impact of the transport method on cEV-associated protein composition was heterogeneous and likely donor-specific. Correlation analysis was nonetheless able to detect that vibration dose, shocks, and imparted energy were associated with different terms depending on the transport, namely in C with cytoskeleton-regulated cell organization activity, and in PTS with a release of extracellular vesicles, mainly from organelle origin, and specifically from mitochondrial structures. Feature selection algorithm identified proteins which, when considered together with the correlated protein-protein interaction network, could be viewed as surrogates of network clusters.