Impact of a Single Oral Acute Dose of Aflatoxin B₁ on Liver Function/Cytokines and the Lymphoproliferative Response in C57Bl/6 Mice

Aflatoxin B₁ (AFB₁), a mycotoxin found in food and feed, exerts harmful effects on humans and animals. The liver is the earliest target of AFB₁, and its effects have been evaluated in animal models exposed to acute or chronic doses. Considering the possibility of sporadic ingestion of AFB₁-contamina...

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Bibliographic Details
Published in:Toxins 2017-11, Vol.9 (11), p.374
Main Authors: Ishikawa, Angélica Tieme, Hirooka, Elisa Yoko, Alvares E Silva, Paula Leonello, Bracarense, Ana Paula Frederico Rodrigues Loureiro, Flaiban, Karina Keller Marques da Costa, Akagi, Claudia Yuri, Kawamura, Osamu, Costa, Marcio Carvalho da, Itano, Eiko Nakagawa
Format: Article
Language:English
Subjects:
Aflatoxin B1
Aflatoxins
Animal models
Body weight
Concanavalin A
Cytokines
Food contamination
Food contamination & poisoning
Histopathology
immunosuppression
inflammatory response
Ingestion
Interferon
Interleukin 17
Interleukin 4
Lesions
Liver
Lymphocytes
Mice
Mitogens
mycotoxin
Mycotoxins
Spleen
γ-Interferon
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Summary:Aflatoxin B₁ (AFB₁), a mycotoxin found in food and feed, exerts harmful effects on humans and animals. The liver is the earliest target of AFB₁, and its effects have been evaluated in animal models exposed to acute or chronic doses. Considering the possibility of sporadic ingestion of AFB₁-contaminated food, this study investigated the impact of a single oral dose of AFB₁ on liver function/cytokines and the lymphoproliferative response in mice. C57BL/6 mice were treated with a single oral AFB₁ dose (44, 442 or 663 μg AFB₁/kg of body weight) on the first day. Liver function (ALT, γ-GT, and total protein), cytokines (IL-4, IFN-γ, and IL-17), histopathology, and the spleen lymphoproliferative response to mitogens were evaluated on the 5th day. Although AFB₁ did not produce any significant changes in the biochemical parameters, 663 μg AFB₁/kg-induced hepatic upregulation of IL-4 and IFN-γ, along with liver tissue injury and suppression of the lymphoproliferative response to ConA ( < 0.05). In conclusion, a single oral dose of AFB₁ exposure can induce liver tissue lesions, liver cytokine modulation, and immune suppression in C57BL/6 mice.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins9110374