Proteomic Analysis of Circulating Extracellular Vesicles Identifies Potential Biomarkers for Lymph Node Metastasis in Oral Tongue Squamous Cell Carcinoma

Lymph node metastasis is the most reliable indicator of a poor prognosis for patients with oral tongue cancers. Currently, there are no biomarkers to predict whether a cancer will spread in the future if it has not already spread at the time of diagnosis. The aim of this study was to quantitatively...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2021-08, Vol.10 (9), p.2179
Main Authors: Qu, Xinyu, Leung, Thomas C. N., Ngai, Sai-Ming, Tsai, Sau-Na, Thakur, Abhimanyu, Li, Wing-Kar, Lee, Youngjin, Leung, Leanne, Ng, Tung-Him, Yam, Judy, Lan, Linlin, Lau, Eric H. L., Wong, Eddy W. Y., Chan, Jason Y. K., Meehan, Katie
Format: Article
Language:English
Subjects:
Biomarkers
Cancer therapies
Cloning
Extracellular vesicles
High-performance liquid chromatography
Kinases
Labeling
lymph node metastases
Lymph nodes
Lymphatic system
Mass spectroscopy
Medical prognosis
Metastases
Metastasis
Microscopy
Oral cancer
Oral carcinoma
oral tongue squamous cell carcinoma
Peptides
Plasma
Proteins
Proteomes
proteomics
Squamous cell carcinoma
Tongue
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Summary:Lymph node metastasis is the most reliable indicator of a poor prognosis for patients with oral tongue cancers. Currently, there are no biomarkers to predict whether a cancer will spread in the future if it has not already spread at the time of diagnosis. The aim of this study was to quantitatively profile the proteomes of extracellular vesicles (EVs) isolated from blood samples taken from patients with oral tongue squamous cell carcinoma with and without lymph node involvement and non-cancer controls. EVs were enriched using size exclusion chromatography (SEC) from pooled plasma samples of patients with non-nodal and nodal oral tongue squamous cell carcinoma (OTSCC) and non-cancer controls. Protein cargo was quantitatively profiled using isobaric labelling (iTRAQ) and two-dimensional high-performance liquid chromatography followed by tandem mass spectrometry. We identified 208 EV associated proteins and, after filtering, generated a short list of 136 proteins. Over 85% of the EV-associated proteins were associated with the GO cellular compartment term “extracellular exosome”. Comparisons between non-cancer controls and oral tongue squamous cell carcinoma with and without lymph node involvement revealed 43 unique candidate EV-associated proteins with deregulated expression patterns. The shortlisted EV associated proteins described here may be useful discriminatory biomarkers for differentiating OTSCC with and without nodal disease or non-cancer controls.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells10092179