Demonstration of reciprocal diurnal variation in human serum T3 and rT3 concentration demonstrated by mass spectrometric analysis and establishment of thyroid hormone reference intervals

Background: There has been a wide range of reference intervals proposed in previous literature for thyroid hormones due to large between-assay variability of immunoassays, as well as lack of correction for collection time. We provided the diurnal reference intervals for five thyroid hormones, namely...

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Bibliographic Details
Published in:Therapeutic advances in endocrinology and metabolism 2020, Vol.11, p.2042018820922688-2042018820922688
Main Authors: Sun, Qian, Avallone, Lívia, Stolze, Brian, Araque, Katherine A., Özarda, Yesim, Jonklaas, Jacqueline, Parikh, Toral, Welsh, Kerry, Masika, Likhona, Soldin, Steven J.
Format: Article
Language:English
Subjects:
Collection
Diurnal variations
Gender aspects
Hormones
Immunoassays
Intervals
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Original Research
Sex differences
Standardization
Thyroid
Thyroid diseases
Thyroid gland
Thyroid hormones
Thyroxine
Triiodothyronine
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Summary:Background: There has been a wide range of reference intervals proposed in previous literature for thyroid hormones due to large between-assay variability of immunoassays, as well as lack of correction for collection time. We provided the diurnal reference intervals for five thyroid hormones, namely total thyroxine (TT4), total triiodothyronine (TT3), free thyroxine (FT4), free triiodothyronine (FT3), and reverse T3 (rT3), measured in serum samples of healthy participants using a liquid chromatography/tandem mass spectrometry (LC-MS/MS) method. Methods: Couplet serum samples (a.m. and p.m.) were collected from 110 healthy females and 49 healthy males. Healthy volunteers were recruited from four participating centers between 2016 and 2018. Measurements of thyroid hormones were obtained by LC-MS/MS analysis. Results: Our study revealed significant uptrend in AM to PM FT4 (p 
ISSN:2042-0188
2042-0196
DOI:10.1177/2042018820922688