Towards effectiveness of cell free DNA based liquid biopsy in head and neck squamous cell carcinoma

Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such...

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Bibliographic Details
Published in:Scientific reports 2024-01, Vol.14 (1), p.2251-11, Article 2251
Main Authors: Kowal-Wisniewska, Ewelina, Jaskiewicz, Katarzyna, Bartochowska, Anna, Kiwerska, Katarzyna, Ustaszewski, Adam, Gorecki, Tomasz, Giefing, Maciej, Paluszczak, Jaroslaw, Wierzbicka, Malgorzata, Jarmuz-Szymczak, Malgorzata
Format: Article
Language:English
Subjects:
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Age
Biomarkers, Tumor - genetics
Biopsy
Body fluids
Cell-Free Nucleic Acids - genetics
Head & neck cancer
Head and neck carcinoma
Head and Neck Neoplasms - diagnosis
Head and Neck Neoplasms - genetics
Head and Neck Neoplasms - surgery
Humanities and Social Sciences
Humans
Liquid Biopsy
multidisciplinary
Nucleic acids
Science
Science (multidisciplinary)
Specimen Handling
Squamous cell carcinoma
Squamous Cell Carcinoma of Head and Neck - genetics
Squamous Cell Carcinoma of Head and Neck - surgery
Tumors
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Summary:Liquid biopsy is a minimally invasive procedure, that uses body fluids sampling to detect and characterize cancer fingerprints. It is of great potential in oncology, however there are challenges associated with the proper handling of liquid biopsy samples that need to be addressed to implement such analysis in patients’ care. Therefore, in this study we performed optimization of pre-analytical conditions and detailed characterization of cfDNA fraction (concentration, length, integrity score) in surgically treated HNSCC patients (n = 152) and healthy volunteers (n = 56). We observed significantly higher cfDNA concentration in patients compared to healthy controls (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-52031-5