Aerobic Exercise Training Improves Microvascular Function and Oxidative Stress Parameters in Diet-Induced Type 2 Diabetic Mice

• Published in: Diabetes, metabolic syndrome and obesity metabolic syndrome and obesity, 2022-10, Vol.15, p.2991-3005
• Main Authors: Rodrigues, Karine Lino, Da Silva, Vivian Vieira Dias, da Silva Nunes Pereira, Evelyn Goulart, Silvares, Raquel Rangel, Araujo, Beatriz Peres de, Flores, Edgar Eduardo Ilaquita, Ramos, Isalira Peroba, Borges, Juliana Pereira, Fernandes-Santos, Caroline, Daliry, Anissa
• Format: Article
• Language: English
• Subjects: Abdomen; Adipose tissues; Aerobics; age-rage; Antioxidants; Blood pressure; Body fat; Carbohydrates; Diabetes; Diet; Diet therapy; Exercise; Fitness equipment; Fitness training programs; Glucose; Hyperglycemia; Insulin resistance; Laboratory animals; Lasers; Liver; Liver diseases; Metabolism; Mice; microcirculation; Microscopy; Original Research; Oxidative stress; Physical fitness; physical training; T cells; Type 2 diabetes; Ultrasonic imaging; Velocity
• ISSN: 1178-7007; 1178-7007
• DOI: 10.2147/DMSO.S365496

Purpose: Type 2 diabetic (T2D) patients have liver and adipose tissue microcirculation disturbances associated with metabolic dysfunction and disease progression. However, the potential role of aerobic training on hepatic and white adipose tissue (WAT) microcirculation and the underlying mechanisms have not been elucidated to date. Therefore, we investigated the role of aerobic training on liver and WAT microcirculation and AGE-RAGE modulation in T2D mice. Methods: The control group (CTL) was fed standard chow, and T2D was induced by feeding male C57BL/6 a high-fat, high-carbohydrate diet for 24 weeks. In the following 12 weeks, mice underwent aerobic training (CTL EX and T2D EX groups), or were kept sedentary (CTL and T2D groups). We assessed metabolic parameters, biochemical markers, oxidative damage, the AGE-RAGE axis, hepatic steatosis, hepatic stellate cells activation (HSC) and liver and WAT microcirculation. Results: Hepatic microcirculation was improved in T2D EX mice which were associated with improvements in body, liver and fat mass, blood pressure, hepatic steatosis and fibrosis, and decreased HSC and AGE-RAGE activation. In contrast, improvement in WAT microcirculation, that is, decreased leukocyte recruitment and increased perfusion, was associated with increased catalase antioxidant activity. Conclusion: Physical training improves hepatic and adipose tissue microcirculatory dysfunction associated with T2D, likely due to downregulation of AGE-RAGE axis, decreased HSC activation and increased antioxidant activity. Keywords: microcirculation, physical training, AGE-RAGE, hyperglycemia