Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758)

Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758). Chelonibia testudinaria appea...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2016-05, Vol.4, p.e2019-e2019, Article e2019
Main Authors: Ewers-Saucedo, Christine, Zardus, John D, Wares, John P
Format: Article
Language:English
Subjects:
Ecology
Evolutionary Studies
Genomics
Homozygote excess
HWE
Microsatellite markers
MiSeq
Molecular Biology
PALFinder
R environment
Zoology
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Summary:Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758). Chelonibia testudinaria appears to be a host generalist, and has an unusual sexual system, androdioecy. Genetic studies on host specificity and mating behavior are impeded by the lack of fine-scale, highly variable markers, such as microsatellite markers. In the present study, we discovered thousands of new microsatellite loci from next-generation sequencing data, and characterized 12 loci thoroughly. We conclude that 11 of these loci will be useful markers in future ecological and evolutionary studies on C. testudinaria.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.2019