Differential expression of intestinal genes in necrotic enteritis challenged broiler chickens with 2 different Clostridium perfringens strains

The primary cause of necrotic enteritis (NE) disease in chickens is the NetB-positive Clostridium perfringens bacterium. Many factors are known to affect the severity of NE in the challenge models of broiler chickens, and one of these factors is the virulence of C. perfringens strain. This study was...

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Bibliographic Details
Published in:Poultry science 2021-03, Vol.100 (3), p.100886, Article 100886
Main Authors: Gharib-Naseri, Kosar, Kheravii, Sarbast, Keerqin, Chake, Swick, Robert A., Choct, Mingan, Wu, Shu-Biao
Format: Article
Language:English
Subjects:
Animal Feed - analysis
Animals
broiler
Chickens - genetics
Clostridium Infections - microbiology
Clostridium Infections - physiopathology
Clostridium Infections - veterinary
Clostridium perfringens
Clostridium perfringens - classification
Clostridium perfringens - pathogenicity
Enteritis - microbiology
Enteritis - physiopathology
Enteritis - veterinary
gene expression
Gene Expression Profiling
Gene Expression Regulation
Immunology, Health and Disease
Intestines - microbiology
Intestines - physiology
Male
necrotic enteritis
Poultry Diseases - microbiology
Poultry Diseases - physiopathology
strains
virulence
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Summary:The primary cause of necrotic enteritis (NE) disease in chickens is the NetB-positive Clostridium perfringens bacterium. Many factors are known to affect the severity of NE in the challenge models of broiler chickens, and one of these factors is the virulence of C. perfringens strain. This study was conducted to evaluate the effect of 2 pathogenic C. perfringens strains in a NE challenge model on gut health and mRNA expression of genes encoding apoptosis, tight junction, immunity, and nutrient transporters in broilers. Day-old Ross-308 male broilers (n = 468) were allocated in a 2 × 3 factorial arrangement of treatments with in-feed antibiotics (no or yes) and challenge (Non, C. perfringens strain NE18, and C. perfringens strain NE36) as the factors. The birds in the challenged groups were inoculated with Eimeria species on day 9 and with a fresh suspension of C. perfringens NE18 or NE36 on day 14 and 15. Sample collection was performed on 2 birds of each pen on day 16. Necrotic enteritis challenge, impaired feed conversion ratio during day 0 to 16 compared with the control group where the effect of the NE36 challenge was more severe than that with NE18 (P 
ISSN:0032-5791
1525-3171
DOI:10.1016/j.psj.2020.11.063