Reporting an Experience: Improving the Feulgen Staining Technique for Better Visualizing of Nucleus

Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to...

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Bibliographic Details
Published in:Iranian journal of pathology 2018, Vol.13 (1), p.106-107
Main Author: Jalayer Naderi, Noushin
Format: Article
Language:English
Subjects:
feulgen staining
Letter to Editor
nuclear abnormalities
nucleus
staining technique
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Summary:Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to false-positive or false-negative results. From self-experience, immersing the stained slides in hematoxylin for 3-5 minutes in final step after staining the slides with light green, provides a greater ground contrast and very better visualizing of nucleus. By this method, a clear distinction can be made between micronuclei and other structures suggestive of a micronucleus.
ISSN:1735-5303
2345-3656
DOI:10.30699/ijp.13.1.106