Phytochemical Profiling of Methanolic Fruit Extract of Gardenia latifolia Ait. by LC-MS/MS Analysis and Evaluation of Its Antioxidant and Antimicrobial Activity

Ait. (Rubiaceae) is also known as Indian Boxwood is a small deciduous tree often growing in southern states of India. In the present study, phytochemical profiling of methanolic extract of fruits were carried out using FTIR and LC-MS/MS analysis. Besides, its antioxidant and antimicrobial potential...

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Bibliographic Details
Published in:Plants (Basel) 2021-03, Vol.10 (3), p.545
Main Authors: Reddy, Y Mohan, Kumar, S P Jeevan, Saritha, K V, Gopal, P, Reddy, T Madhusudana, Simal-Gandara, Jesus
Format: Article
Language:English
Subjects:
Acids
Antibacterial activity
Antimicrobial activity
Antimicrobial agents
antioxidant activity
Antioxidants
Bacteria
Cancer
Chlorogenic acid
Deciduous trees
Diabetes
differential pulse voltammetry
E coli
Flavonoids
Food industry
Free radicals
Fruits
G. latifolia
Gardenia
Gardens & gardening
Klebsiella
LC-MS/MS analysis
Metabolites
Minimum inhibitory concentration
Natural products
Oxidation
Phenols
Phytochemicals
Pseudomonas aeruginosa
Quercetin
Quinic acid
Rutin
Solvents
Voltammetry
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Summary:Ait. (Rubiaceae) is also known as Indian Boxwood is a small deciduous tree often growing in southern states of India. In the present study, phytochemical profiling of methanolic extract of fruits were carried out using FTIR and LC-MS/MS analysis. Besides, its antioxidant and antimicrobial potential have been analysed using DPPH activity, differential pulse voltammetry and resazurin microtiter assay, respectively. Phytochemical profiling revealed the presence of 22 major diversified compounds and main were 3-caffeoyl quinic acid (chlorogenic acid), 3,4-Di-O-caffeoyl quinic acid, 6-O-trans-feruloylgenipin gentiobioside, 10-(6-O-trans sinapoyl glucopyranosyl) gardendiol, isoquercitrin, scortechinones, secaubryenol, iridoids and quercetin 3-rutinoside (rutin). The extract showed antioxidant activity (IC = 65.82) and powerful antibacterial activity with lowest minimum inhibitory concentration against Gram-positive (15.62 µg/µL), (31.25 µg/µL) than gram negative (62.5 µg/µL), (62.5 µg/µL), (31.25 µg/µL). This study shows that the fruits of have tremendous potential to be used in food industries, phyto-therapeutics and cosmetic industries.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10030545