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Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh
One of the most significant public health concerns in today's world is the persistent upsurge of infections caused by multidrug resistant bacteria. As a result, clinicians are being forced to intervene with either less effective backup drugs or ones with substantial side-effects. Colistin is a...
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| Published in: | Gut pathogens 2020-01, Vol.12 (1), p.5-5, Article 5 |
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| creator | Johura, Fatema-Tuz Tasnim, Jarin Barman, Indrajeet Biswas, Sahitya Ranjan Jubyda, Fatema Tuz Sultana, Marzia George, Christine Marie Camilli, Andrew Seed, Kimberley D Ahmed, Niyaz Alam, Munirul |
| description | One of the most significant public health concerns in today's world is the persistent upsurge of infections caused by multidrug resistant bacteria. As a result, clinicians are being forced to intervene with either less effective backup drugs or ones with substantial side-effects. Colistin is a last resort antimicrobial agent for the treatment of infections caused by multi-drug resistant gram-negative bacteria.
(n = 65) isolated from street food (n = 20), hand rinse (n = 15), surface water (n = 10), and healthy human stool (n = 20) were tested for colistin resistance gene
and response to antimicrobial agents. Antimicrobial resistance genes and virulence genes were detected by employing polymerase chain reaction. DNA fingerprinting of the strains were determined by pulsed-field gel electrophoresis.
Screening of
allowed us to confirm colistin resistance marker gene
1 in 13 strains (street food, n = 4; hand rinse, n = 2; surface water, n = 4; and stool, n = 3); and two of these
strains carrying
-1 harbored
gene encoding extended spectrum beta lactamase. Antibiotic assay results revealed all 13
strains carrying
-1 to be multi-drug resistant (MDR), including to colistin. The minimum inhibitory concentration (MIC) for colistin ranged from 2 to 6 μg/ml. DNA sequencing confirmed homogeneity of the nucleotide sequence for
, but the
strains were heterogenous, as confirmed by pulsed-field gel electrophoresis suggesting horizontal transmission of colistin resistance in Bangladesh.
Widespread dissemination of
strains carrying
-1 encoding resistance to colistin in the present study is alarming as this is the last resort drug for the treatment of infections caused by MDR gram-negative bacteria resistant to almost all drugs used commonly. |
| doi_str_mv | 10.1186/s13099-020-0345-2 |
| format | article |
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(n = 65) isolated from street food (n = 20), hand rinse (n = 15), surface water (n = 10), and healthy human stool (n = 20) were tested for colistin resistance gene
and response to antimicrobial agents. Antimicrobial resistance genes and virulence genes were detected by employing polymerase chain reaction. DNA fingerprinting of the strains were determined by pulsed-field gel electrophoresis.
Screening of
allowed us to confirm colistin resistance marker gene
1 in 13 strains (street food, n = 4; hand rinse, n = 2; surface water, n = 4; and stool, n = 3); and two of these
strains carrying
-1 harbored
gene encoding extended spectrum beta lactamase. Antibiotic assay results revealed all 13
strains carrying
-1 to be multi-drug resistant (MDR), including to colistin. The minimum inhibitory concentration (MIC) for colistin ranged from 2 to 6 μg/ml. DNA sequencing confirmed homogeneity of the nucleotide sequence for
, but the
strains were heterogenous, as confirmed by pulsed-field gel electrophoresis suggesting horizontal transmission of colistin resistance in Bangladesh.
Widespread dissemination of
strains carrying
-1 encoding resistance to colistin in the present study is alarming as this is the last resort drug for the treatment of infections caused by MDR gram-negative bacteria resistant to almost all drugs used commonly.</description><identifier>ISSN: 1757-4749</identifier><identifier>EISSN: 1757-4749</identifier><identifier>DOI: 10.1186/s13099-020-0345-2</identifier><identifier>PMID: 32002025</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Antibacterial agents ; Antibiotics ; Antimicrobial agents ; Antimicrobial resistance ; Bacteria ; Bacterial infections ; Bangladesh ; Base sequence ; Beta lactamases ; Care and treatment ; Colistin ; Deoxyribonucleic acid ; DNA ; DNA fingerprinting ; DNA sequencing ; Drug resistance ; E coli ; Electrophoresis ; ESBL ; Escherichia coli ; Food ; Gel electrophoresis ; Genes ; Gram-negative bacteria ; Health aspects ; Health care ; Horizontal transmission ; Infection ; Infections ; Infectious diseases ; Laboratories ; mcr-1 ; Methylene blue ; Microbial drug resistance ; Minimum inhibitory concentration ; Minimum inhibitory concentration (MIC) ; Multi-drug resistant (MDR) ; Multidrug resistance ; Nucleotide sequence ; Polymerase chain reaction ; Public health ; Public health movements ; Pulsed-field gel electrophoresis ; Strains (organisms) ; Surface water ; Virulence ; Virulence (Microbiology) ; Water ; Water resources ; β Lactamase</subject><ispartof>Gut pathogens, 2020-01, Vol.12 (1), p.5-5, Article 5</ispartof><rights>The Author(s) 2020.</rights><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c629t-5801f8e29045048a9842e249ae32259ce19a9f3f524ea80fc3eed35a7a20fb4c3</citedby><cites>FETCH-LOGICAL-c629t-5801f8e29045048a9842e249ae32259ce19a9f3f524ea80fc3eed35a7a20fb4c3</cites><orcidid>0000-0001-9157-5560</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986151/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2357786960?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32002025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johura, Fatema-Tuz</creatorcontrib><creatorcontrib>Tasnim, Jarin</creatorcontrib><creatorcontrib>Barman, Indrajeet</creatorcontrib><creatorcontrib>Biswas, Sahitya Ranjan</creatorcontrib><creatorcontrib>Jubyda, Fatema Tuz</creatorcontrib><creatorcontrib>Sultana, Marzia</creatorcontrib><creatorcontrib>George, Christine Marie</creatorcontrib><creatorcontrib>Camilli, Andrew</creatorcontrib><creatorcontrib>Seed, Kimberley D</creatorcontrib><creatorcontrib>Ahmed, Niyaz</creatorcontrib><creatorcontrib>Alam, Munirul</creatorcontrib><title>Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh</title><title>Gut pathogens</title><addtitle>Gut Pathog</addtitle><description>One of the most significant public health concerns in today's world is the persistent upsurge of infections caused by multidrug resistant bacteria. As a result, clinicians are being forced to intervene with either less effective backup drugs or ones with substantial side-effects. Colistin is a last resort antimicrobial agent for the treatment of infections caused by multi-drug resistant gram-negative bacteria.
(n = 65) isolated from street food (n = 20), hand rinse (n = 15), surface water (n = 10), and healthy human stool (n = 20) were tested for colistin resistance gene
and response to antimicrobial agents. Antimicrobial resistance genes and virulence genes were detected by employing polymerase chain reaction. DNA fingerprinting of the strains were determined by pulsed-field gel electrophoresis.
Screening of
allowed us to confirm colistin resistance marker gene
1 in 13 strains (street food, n = 4; hand rinse, n = 2; surface water, n = 4; and stool, n = 3); and two of these
strains carrying
-1 harbored
gene encoding extended spectrum beta lactamase. Antibiotic assay results revealed all 13
strains carrying
-1 to be multi-drug resistant (MDR), including to colistin. The minimum inhibitory concentration (MIC) for colistin ranged from 2 to 6 μg/ml. DNA sequencing confirmed homogeneity of the nucleotide sequence for
, but the
strains were heterogenous, as confirmed by pulsed-field gel electrophoresis suggesting horizontal transmission of colistin resistance in Bangladesh.
Widespread dissemination of
strains carrying
-1 encoding resistance to colistin in the present study is alarming as this is the last resort drug for the treatment of infections caused by MDR gram-negative bacteria resistant to almost all drugs used commonly.</description><subject>Antibacterial agents</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Bangladesh</subject><subject>Base sequence</subject><subject>Beta lactamases</subject><subject>Care and treatment</subject><subject>Colistin</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>DNA sequencing</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Electrophoresis</subject><subject>ESBL</subject><subject>Escherichia coli</subject><subject>Food</subject><subject>Gel electrophoresis</subject><subject>Genes</subject><subject>Gram-negative bacteria</subject><subject>Health aspects</subject><subject>Health care</subject><subject>Horizontal transmission</subject><subject>Infection</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Laboratories</subject><subject>mcr-1</subject><subject>Methylene blue</subject><subject>Microbial drug resistance</subject><subject>Minimum inhibitory concentration</subject><subject>Minimum inhibitory concentration (MIC)</subject><subject>Multi-drug resistant (MDR)</subject><subject>Multidrug resistance</subject><subject>Nucleotide sequence</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>Public health movements</subject><subject>Pulsed-field gel electrophoresis</subject><subject>Strains (organisms)</subject><subject>Surface water</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Water</subject><subject>Water resources</subject><subject>β 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Central</general><general>BMC</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-9157-5560</orcidid></search><sort><creationdate>20200127</creationdate><title>Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh</title><author>Johura, Fatema-Tuz ; Tasnim, Jarin ; Barman, Indrajeet ; Biswas, Sahitya Ranjan ; Jubyda, Fatema Tuz ; Sultana, Marzia ; George, Christine Marie ; Camilli, Andrew ; Seed, Kimberley D ; Ahmed, Niyaz ; Alam, Munirul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c629t-5801f8e29045048a9842e249ae32259ce19a9f3f524ea80fc3eed35a7a20fb4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibacterial agents</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Bangladesh</topic><topic>Base sequence</topic><topic>Beta lactamases</topic><topic>Care and treatment</topic><topic>Colistin</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA fingerprinting</topic><topic>DNA sequencing</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Electrophoresis</topic><topic>ESBL</topic><topic>Escherichia coli</topic><topic>Food</topic><topic>Gel electrophoresis</topic><topic>Genes</topic><topic>Gram-negative bacteria</topic><topic>Health aspects</topic><topic>Health care</topic><topic>Horizontal transmission</topic><topic>Infection</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Laboratories</topic><topic>mcr-1</topic><topic>Methylene blue</topic><topic>Microbial drug resistance</topic><topic>Minimum inhibitory concentration</topic><topic>Minimum inhibitory concentration (MIC)</topic><topic>Multi-drug resistant (MDR)</topic><topic>Multidrug resistance</topic><topic>Nucleotide sequence</topic><topic>Polymerase chain reaction</topic><topic>Public health</topic><topic>Public health movements</topic><topic>Pulsed-field gel electrophoresis</topic><topic>Strains (organisms)</topic><topic>Surface water</topic><topic>Virulence</topic><topic>Virulence (Microbiology)</topic><topic>Water</topic><topic>Water resources</topic><topic>β 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Pathog</addtitle><date>2020-01-27</date><risdate>2020</risdate><volume>12</volume><issue>1</issue><spage>5</spage><epage>5</epage><pages>5-5</pages><artnum>5</artnum><issn>1757-4749</issn><eissn>1757-4749</eissn><abstract>One of the most significant public health concerns in today's world is the persistent upsurge of infections caused by multidrug resistant bacteria. As a result, clinicians are being forced to intervene with either less effective backup drugs or ones with substantial side-effects. Colistin is a last resort antimicrobial agent for the treatment of infections caused by multi-drug resistant gram-negative bacteria.
(n = 65) isolated from street food (n = 20), hand rinse (n = 15), surface water (n = 10), and healthy human stool (n = 20) were tested for colistin resistance gene
and response to antimicrobial agents. Antimicrobial resistance genes and virulence genes were detected by employing polymerase chain reaction. DNA fingerprinting of the strains were determined by pulsed-field gel electrophoresis.
Screening of
allowed us to confirm colistin resistance marker gene
1 in 13 strains (street food, n = 4; hand rinse, n = 2; surface water, n = 4; and stool, n = 3); and two of these
strains carrying
-1 harbored
gene encoding extended spectrum beta lactamase. Antibiotic assay results revealed all 13
strains carrying
-1 to be multi-drug resistant (MDR), including to colistin. The minimum inhibitory concentration (MIC) for colistin ranged from 2 to 6 μg/ml. DNA sequencing confirmed homogeneity of the nucleotide sequence for
, but the
strains were heterogenous, as confirmed by pulsed-field gel electrophoresis suggesting horizontal transmission of colistin resistance in Bangladesh.
Widespread dissemination of
strains carrying
-1 encoding resistance to colistin in the present study is alarming as this is the last resort drug for the treatment of infections caused by MDR gram-negative bacteria resistant to almost all drugs used commonly.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>32002025</pmid><doi>10.1186/s13099-020-0345-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9157-5560</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Gut pathogens, 2020-01, Vol.12 (1), p.5-5, Article 5 |
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| language | eng |
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| source | Open Access: PubMed Central; Publicly Available Content Database |
| subjects | Antibacterial agents Antibiotics Antimicrobial agents Antimicrobial resistance Bacteria Bacterial infections Bangladesh Base sequence Beta lactamases Care and treatment Colistin Deoxyribonucleic acid DNA DNA fingerprinting DNA sequencing Drug resistance E coli Electrophoresis ESBL Escherichia coli Food Gel electrophoresis Genes Gram-negative bacteria Health aspects Health care Horizontal transmission Infection Infections Infectious diseases Laboratories mcr-1 Methylene blue Microbial drug resistance Minimum inhibitory concentration Minimum inhibitory concentration (MIC) Multi-drug resistant (MDR) Multidrug resistance Nucleotide sequence Polymerase chain reaction Public health Public health movements Pulsed-field gel electrophoresis Strains (organisms) Surface water Virulence Virulence (Microbiology) Water Water resources β Lactamase |
| title | Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh |
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