Polymorphism of insulin-like growth factor 1 gene and its relationship with reproductive performances and milk yield in Sarda dairy sheep

• Published in: Veterinary and animal science 2020-06, Vol.9, p.100084-100084, Article 100084
• Main Authors: Sebastiano, Luridiana, Consuelo, Mura Maria, Veronica, Di Stefano Maria, Luisa, Pulinas, Giovanni, Cosso, Michella, Nehme, Vincenzo, Carcangiu
• Format: Article
• Language: English
• Subjects: IGF-1 gene polymorphisms; Reproductive performances; Sarda sheep
• ISSN: 2451-943X; 2451-943X
• DOI: 10.1016/j.vas.2019.100084

•IGF-1 gene plays an important role in regulating reproduction in ruminants.•Here three SNPs were found in the IGF-I gene in Sarda sheep.•The found SNPs were related to reproductive performance and milk yield.•This results could be used to improve the reproductive performances and milk yield in Sarda sheep. The aim of this research was to detect polymorphisms within the IGF-I gene in dairy sheep and to verify their influence on milk yield and reproductive performances. Four hundred Sarda ewes in their second lactation were selected from 2 farms. Their reproductive traits (fertility rate, interval in days from ram introduction to lambing and litter size) and milk yield were recorded, from the second to the fourth lactation. DNA was extracted from individual blood samples and subjected to amplification and sequencing of the IGF-I gene 5′ UTR and Exon 3 regions. Three polymorphic sites were recorded at positions g184028491C>G and g184028489C>T of the 5′UTR, and g184023223G>A of the Exon 3. The C allele at position g184028491 showed a significant association with higher fertility rate (P