A simple urine test by 3D‐plus‐3D immunoassay guides precise in vitro cancer diagnosis

Although a variety of urinary cancer markers are available for in vitro diagnosis, inherent problems of urine environment—containing various inorganic/organic ions/molecules that vary in concentration over a 20‐fold range or more and significantly attenuate antibody avidity for markers—render conven...

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Bibliographic Details
Published in:Bioengineering & translational medicine 2023-05, Vol.8 (3), p.e10489-n/a
Main Authors: Kim, Hye Hyun, Moon, Ok Jeong, Seol, Yong Hwan, Lee, Jeewon
Format: Article
Language:English
Subjects:
3D‐IgG probes
3D‐plus‐3D immunoassay
Antibodies
Antigens
Diagnosis
Disease
Hepatitis B
Immunoassay
in vitro cancer diagnosis
Lung cancer
Medical diagnosis
Microscopy
Prostate cancer
Proteins
signal self‐enhancement
Steric hindrance
Urine
urine test
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Summary:Although a variety of urinary cancer markers are available for in vitro diagnosis, inherent problems of urine environment—containing various inorganic/organic ions/molecules that vary in concentration over a 20‐fold range or more and significantly attenuate antibody avidity for markers—render conventional immunoassays unsuitable, remaining unresolved and a major challenge. Here we developed a 3D‐plus‐3D (3p3) immunoassay method, based on a single‐step urinary marker detection by 3D‐antibody probes, which are free of steric hindrance and capable of omnidirectional capture of markers in a 3D solution. The 3p3 immunoassay showed an excellent performance in the diagnosis of prostate cancer (PCa) through detecting PCa‐specific urinary engrailed‐2 protein, demonstrating 100% sensitivity and 100% specificity with the urine specimens of PCa‐related and other related disease patients and healthy individuals. This innovative approach holds a great potential in opening up a novel clinical route for precise in vitro cancer diagnosis and also pushing urine immunoassay closer to more widespread adoption.
ISSN:2380-6761
2380-6761
DOI:10.1002/btm2.10489