Molecular characterization of 'sweet' cassavas (Manihot esculenta) from a germplasm bank in Brazilian Eastern Amazonia

Abstract Genetic variability of a set of 81 accessions of 'sweet' cassavas (Manihot esculenta) collected mostly in the North region of Brazil was investigated with nine microsatellite loci. All loci were polymorphic, with mean of 6.33 alleles per locus. Analyses indicated that 35 multiloci...

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Published in:Crop breeding and applied biotechnology 2016-03, Vol.16 (1), p.28-34
Main Authors: Cunha, Elisa Ferreira Moura, Silva, Carlos Rogério de Sousa, Albuquerque, Paulo Sérgio Bevilaqua de, Ramalho, Girena Fernandes, Pontes, Lígia Cristine Gonçalves, Farias Neto, João Tomé de
Format: Article
Language:English
Subjects:
AGRONOMY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
duplicates
Euphorbiaceae
Genetic resources
Germplasm
microsatellite markers
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Summary:Abstract Genetic variability of a set of 81 accessions of 'sweet' cassavas (Manihot esculenta) collected mostly in the North region of Brazil was investigated with nine microsatellite loci. All loci were polymorphic, with mean of 6.33 alleles per locus. Analyses indicated that 35 multiloci profiles were represented by a single accession, and 46 showed non-unique profiles, represented by eleven genotypes. Forty-six different multiloci profiles were detected. Most of the putative duplicated accessions were collected in different locations. After the removal of putative duplicated genotypes, genetic parameters were estimated and expected heterozygosity was high (HE=0.73), indicating genetic variability. Structure analysis of this set of 'sweet' cassavas divided the 46 genotypes into two clusters (K=2), and a few genotypes had mixed ancestry. Results indicated the habit of exchange of materials among farmers of the North region of Brazil, and the genetic variability to be exploited in genetic breeding efforts.
ISSN:1984-7033
1518-7853
1984-7033
DOI:10.1590/1984-70332016v16n1a5