Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury

Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from c...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-07, Vol.19 (7), p.1963
Main Authors: Ohta, Yuki, Takenaga, Mitsuko, Hamaguchi, Akemi, Ootaki, Masanori, Takeba, Yuko, Kobayashi, Tsukasa, Watanabe, Minoru, Iiri, Taroh, Matsumoto, Naoki
Format: Article
Language:English
Subjects:
Adipocytes
Adipose tissue
Adipose Tissue - cytology
adipose-derived stem/stromal cells
Animals
Cell culture
Cell Differentiation
Cell Proliferation
Cell Separation
Cryopreservation
Disease Models, Animal
Female
Growth factors
Hepatocyte growth factor
Hepatocyte Growth Factor - metabolism
Hindlimb
Humans
Laminectomy
Locomotion
Rats
Rats, Sprague-Dawley
Regenerative Medicine
Rodents
Spinal cord injuries
Spinal Cord Injuries - surgery
spinal cord injury
Stromal cells
Stromal Cells - transplantation
tissue bank
Transplantation
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - metabolism
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Summary:Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from cryopreserved adipose tissue with the aim of developing a banking system. ASCs were isolated from fresh and cryopreserved adipose tissue of rats and compared for proliferation (doubling time), differentiation capability (adipocytes), and cytokine (hepatocyte growth factor and vascular endothelial growth factor) secretion. Finally, ASCs (2.5 × 10⁶) were intravenously infused into rats with spinal cord injury, after which hindlimb motor function was evaluated. Isolation and culture of ASCs from cryopreserved adipose tissue were possible, and their characteristics were not significantly different from those of fresh tissue. Transplantation of ASCs derived from cryopreserved tissue significantly promoted restoration of hindlimb movement function in injured model rats. These results indicate that cryopreservation of adipose tissue may be an option for clinical application.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19071963